Development of MRI/NIRF 'activatable' multimodal imaging probe based on iron oxide nanoparticles
- Authors
- Cha, Eui-Joon; Jang, Eue Soon; Sun, In-Cheol; Lee, In Joon; Ko, Jeong Hoon; Kim, Young Il; Kwon, Ick Chan; Kim, Kwangmeyung; Ahn, Cheol-Hee
- Issue Date
- 30-Oct-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- MRI; Optical imaging; Activatable; Dual imaging; Iron oxide; Core-shell nanoparticle
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.155, no.2, pp.152 - 158
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 155
- Number
- 2
- Start Page
- 152
- End Page
- 158
- URI
- https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/20158
- DOI
- 10.1016/j.jconrel.2011.07.019
- ISSN
- 0168-3659
- Abstract
- A fabrication method of Cy5.5-MMP substrate and PEG conjugated iron oxide nanoparticles with thin silica coating (PCM-CS) and its potential as an 'activatable' dual imaging probe for tumor imaging is described in this report. PCM-CS showed an intensity-averaged diameter of 43.1 +/- 6.3 nm by dynamic light scattering without any noticeable aggregation over 7 days. Fluorescence of Cy5.5 on the surface of nanoparticles was fully quenched and the quenching efficiency was 97.2%. PCM-CS showed protease specific fluorescence recovery in vitro caused from the specific peptide cleavage by MMP-2 and the probe displayed the sensitivity on 0.5 nM or less enzyme concentration. Tumor was successfully visualized by NIRF and MRI in vivo by intravenously injected PCM-CS. NIRF signal of tumor was gradually increased up to 12 h post injection and the intensity of tumor was about 3-4 times higher than normal tissue. NIRF signal at MMP-2 inhibitor treated tumor was clearly lower than tumor without inhibitor due to the insufficient peptide cleavage. NIRF signal at excised tumor was 5-10 times stronger than other organs. Noticeable darkening in magnetic resonance image was observed at the tumor region and the image was gradually darkened at 12 h post injection of PCM-CS. The maximum signal difference between tumor region and healthy muscle was 34%. (C) 2011 Elsevier B.V. All rights reserved.
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Collections - Department of Applied Chemistry > 1. Journal Articles
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