Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae Won-Bin | - |
dc.contributor.author | Kim Hee-Joon | - |
dc.contributor.author | 지광환 | - |
dc.date.accessioned | 2022-05-17T02:40:01Z | - |
dc.date.available | 2022-05-17T02:40:01Z | - |
dc.date.created | 2022-05-17 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/21104 | - |
dc.description.abstract | We report the effect of pH on the supramolecular complexation of two biothiols, viz., homocysteine (Hcy) and cysteine (Cys), with cucurbit[7]uril (CB[7]). Under basic pH conditions, Cys did not complex with CB[7], whereas Hcy efficiently complexed with CB[7], as confirmed by 1H NMR spectroscopy and Ellman’s reagent (5,5’-dithio-bis(2-nitrobenzoic acid), DTNB) assay. 1H NMR and Raman spectroscopic studies revealed that, in the absence of CB[7], Hcy auto-oxidized slowly (~36 h) to homocystine (HSSH) under basic pH conditions. However, the rate of Hcy oxidation increased by up to 150 fold in the presence of CB[7], as suggested by the DTNB assay. Thus, supramolecular complexation under basic pH conditions led to the formation of a HSSH-CB[7] complex, and not Hcy- CB[7]. The results indicate that Hcy is rapidly oxidized to HSSH under the catalysis of CB[7], which acts as a reaction chamber, in basic pH conditions. Our studies suggest that Hcy concentration, a risk factor for cardiovascular disease, can be selectively and more easily quantified by supramolecular complexation with CB [7]. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation | - |
dc.title.alternative | Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae Won-Bin | - |
dc.contributor.affiliatedAuthor | Kim Hee-Joon | - |
dc.contributor.affiliatedAuthor | 지광환 | - |
dc.identifier.doi | 10.4014/jmb.2201.01016 | - |
dc.identifier.wosid | 000821788200013 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.32, no.4, pp.514 - 521 | - |
dc.relation.isPartOf | Journal of Microbiology and Biotechnology | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 32 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 514 | - |
dc.citation.endPage | 521 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002834206 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | CYSTEINE | - |
dc.subject.keywordPlus | THIOLS | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | Homocysteine | - |
dc.subject.keywordAuthor | cysteine | - |
dc.subject.keywordAuthor | cucurbit[7]uril | - |
dc.subject.keywordAuthor | homocystine | - |
dc.subject.keywordAuthor | pH effect | - |
dc.subject.keywordAuthor | supramolecular complexation | - |
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