Fragmentation Analysis of rIAPP Monomer, Dimer, and [M rIAPP + M hIAPP ] 5+ Using Collision-Induced Dissociation with Electrospray Ionization Mass Spectrometry
DC Field | Value | Language |
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dc.contributor.author | Jeongmo Kim | - |
dc.contributor.author | 김호태 | - |
dc.date.accessioned | 2023-12-11T18:30:44Z | - |
dc.date.available | 2023-12-11T18:30:44Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.issn | 2093-8950 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/25786 | - |
dc.description.abstract | Collision-induced dissociation (CID) combined with electrospray ionization mass spectrometry (ESI-MS) was used to obtain structural information on rat islet amyloid polypeptide (rIAPP) monomers (M) and dimers (D) observed in the multiply charged state in the MS spectrum. MS/MS analysis indicated that the rIAPP monomers adopt distinct structures depending on the molecular ion charge state. Peptide bond dissociation between L 27 and P 28 was observed in the MS/MS spectra of rIAPP monomers, regardless of the monomer molecular ion charge state. MS/MS analysis of the dimers indicated that D 5+ comprised M 2+ and M 3+ subunits, and that the peptide bond dissociation process between the L 27 and P 28 residues of the monomer subunit was also maintained. The observation of (M+ b 27 ) 4+ and (M+ y 10 ) 3+ fragment ions were deduced to originate from the two differ- ent D 5+ complex geometries, the N-terminal and C-terminal interaction geometries, respectively. The fragmentation pattern of the [M rIAPP + M hIAPP ] 5+ MS/MS spectrum showed that the interaction occurred between the two N-terminal regions of M rIAPP and M hIAPP in the heterogeneous dimer (hetero-dimer) D 5+ structure. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 사단법인 한국질량분석학회 | - |
dc.title | Fragmentation Analysis of rIAPP Monomer, Dimer, and [M rIAPP + M hIAPP ] 5+ Using Collision-Induced Dissociation with Electrospray Ionization Mass Spectrometry | - |
dc.title.alternative | Fragmentation Analysis of rIAPP Monomer, Dimer, and [M rIAPP + M hIAPP ] 5+ Using Collision-Induced Dissociation with Electrospray Ionization Mass Spectrometry | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.5478/MSL.2021.12.4.179 | - |
dc.identifier.wosid | 000752981100010 | - |
dc.identifier.bibliographicCitation | Mass Spectrometry Letters, v.12, no.4, pp 179 - 185 | - |
dc.citation.title | Mass Spectrometry Letters | - |
dc.citation.volume | 12 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 185 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002791555 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | ISLET AMYLOID POLYPEPTIDE | - |
dc.subject.keywordPlus | ATOMIC STRUCTURES | - |
dc.subject.keywordPlus | IAPP | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | AMYLIN | - |
dc.subject.keywordPlus | FIBRILLATION | - |
dc.subject.keywordPlus | FIBRILS | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | ABILITY | - |
dc.subject.keywordAuthor | rat islet amyloid polypeptide (rIAPP) | - |
dc.subject.keywordAuthor | human islet amyloid polypeptide (hIAPP) | - |
dc.subject.keywordAuthor | heterogeneous dimer | - |
dc.subject.keywordAuthor | collision- induced dissociation mass spectrometry (CID-MS) | - |
dc.subject.keywordAuthor | MS/MS | - |
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