Analysis of Amyloid Beta 1-16 (A beta 16) Monomer and Dimer Using Electrospray Ionization Mass Spectrometry with Collision-Induced Dissociation
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyoung Min | - |
dc.contributor.author | Kim, Ho -Tae | - |
dc.date.accessioned | 2023-12-11T19:32:04Z | - |
dc.date.available | 2023-12-11T19:32:04Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.issn | 2093-8950 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/26288 | - |
dc.description.abstract | The monomer and dimer structures of the amyloid fragment A beta(1-16) sequence formed in H2O were investigated using electrospray ionization mass spectrometry (MS) and tandem MS (MS/MS). A beta 16 monomers and dimers were indicated by signals representing multiple proton adduct forms, [monomer+zH](n+) (=Mz+, z = charge state) and [dimer+zH](z+) (=Dz+), in the MS spectrum. Fragment ions of monomers and dimers were observed using collision-induced dissociation MS/MS. Peptide bond dissociation was mostly observed in the D1-D7 and V11-K16 regions of the MS/MS spectra for the monomer (or dimer), regardless of the monomer (or dimer) charge state. Both covalent and non-covalent bond dissociation processes were indicated by the MS/MS results for the dimers. During the non-covalent bond dissociation process, the D3+ dimer complex was separated into two components: the M1+ and M2+ subunits. During the covalent bond dissociation of the D3+ dimer complex, the b and y fragment ions attached to the monomer, (M+b(10-15))(z+) and (M+y(9-15))(z+), were thought to originate from the dissociation of the M2+ monomer component of the (M1++M2+) complex. Two different D3+ complex geometries exist; two distinguished interaction geometries resulting from interactions between the M1+ monomer and two different regions of M2+ (the N-terminus and C-terminus) are proposed. Intricate fragmentation patterns were observed in the MS/MS spectrum of the D5+ complex. The complicated nature of the MS/MS spectrum is attributable to the coexistence of two D5+ configurations, (M1+ +M4+) and (M2+ + M3+), in the A beta 16 solution. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MASS SPECTROMETRY | - |
dc.title | Analysis of Amyloid Beta 1-16 (A beta 16) Monomer and Dimer Using Electrospray Ionization Mass Spectrometry with Collision-Induced Dissociation | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.5478/MSL.2022.13.4.177 | - |
dc.identifier.scopusid | 2-s2.0-85146379586 | - |
dc.identifier.wosid | 000974913300012 | - |
dc.identifier.bibliographicCitation | MASS SPECTROMETRY LETTERS, v.13, no.4, pp 177 - 183 | - |
dc.citation.title | MASS SPECTROMETRY LETTERS | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 177 | - |
dc.citation.endPage | 183 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | OLIGOMERS | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | A-BETA(1-16) | - |
dc.subject.keywordPlus | RESIDUES | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | A beta 16 | - |
dc.subject.keywordAuthor | collision-induced dissociation | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | MS/MS | - |
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