Investigation of the Copper (Cu) Binding Site on the Amyloid beta 1-16 (Aβ16) Monomer and Dimer Using Collision-induced Dissociation with Electrospray Ionization Tandem Mass Spectrometry
DC Field | Value | Language |
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dc.contributor.author | Jang, Ji Won | - |
dc.contributor.author | Lim, Jin Yeong | - |
dc.contributor.author | Kim, Seo Yeon | - |
dc.contributor.author | Kim, Jin Se | - |
dc.contributor.author | Kim, Ho-Tae | - |
dc.date.accessioned | 2024-07-17T08:01:04Z | - |
dc.date.available | 2024-07-17T08:01:04Z | - |
dc.date.issued | 2024-12 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.issn | 2093-8950 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/28796 | - |
dc.description.abstract | The copper ion, Cu(II), binding sites for amyloid fragment AI31-16 (=AI316 ) were investigated to explain the biological activity difference in the AI316 aggregation process. The [M+Cu+(z-2)H](z+) (z = 2, 3 and 4, M = AI316 monomer) and [D+Cu+(z-2)H](z+) (z = 3 and 5, D = AI316 dimer) structures were investigated using electrospray ionization (ESI) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Fragment ions of the [M+Cu+(z-2)H](z+) and [D+Cu+(z-2)H](z+) complexes were observed using collision-induced dissociation MS/MS. Three different fragmentation patterns (fragment "a", "b", and "y" ion series) were observed in the MS/MS spectrum of the (AI316 monomer or dimer-Cu) complex, with the "b" and "y" ion series regularly observed. The "a" ion series was not observed in the MS/MS spectrum of the [M+Cu+2H](4+) complex. In the non-covalent bond dissociation process, the [D+Cu+3H](5+) complex separated into three components ([M+Cu+H](3+), M3+, and M2+), and the [M+Cu](2+) subunit was not observed. The {M + fragment ion of [M+Cu+H](3+)} fragmentation pattern was observed during the covalent bond dissociation of the [D+Cu +3H](5+) complex. The {M + [M+Cu+H](3+)} complex geometry was assumed to be stable in the [D+Cu+3H](5+) complex. The {M + fragment ion of [M+Cu](2+)} fragmentation pattern was also observed in the MS/MS spectrum of the [D+Cu+H](3+) complex. The {M + [y(9)+Cu](1+)} fragment ion was the characteristic fragment ion. The [D+Cu+H](3+) and [D+Cu+3H](5+)complexes were likely to form a monomer-monomer-Cu (M-M-Cu) structure instead of a monomer-Cu-monomer (M-Cu-M) structure. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MASS SPECTROMETRY | - |
dc.title | Investigation of the Copper (Cu) Binding Site on the Amyloid beta 1-16 (Aβ16) Monomer and Dimer Using Collision-induced Dissociation with Electrospray Ionization Tandem Mass Spectrometry | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.5478/MSL.2023.14.4.153 | - |
dc.identifier.scopusid | 2-s2.0-85182632275 | - |
dc.identifier.wosid | 001246226200004 | - |
dc.identifier.bibliographicCitation | MASS SPECTROMETRY LETTERS, v.14, no.4, pp 153 - 159 | - |
dc.citation.title | MASS SPECTROMETRY LETTERS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 153 | - |
dc.citation.endPage | 159 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART003038494 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | A-BETA | - |
dc.subject.keywordPlus | DETACHMENT DISSOCIATION | - |
dc.subject.keywordPlus | REDOX CHEMISTRY | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FRAGMENTATION | - |
dc.subject.keywordAuthor | A beta 16 | - |
dc.subject.keywordAuthor | [A beta 16 monomer-Cu] complex | - |
dc.subject.keywordAuthor | [A beta 16 dimer-Cu] complex | - |
dc.subject.keywordAuthor | collision-induced dissociation (CID) | - |
dc.subject.keywordAuthor | mass spectrometry (MS) | - |
dc.subject.keywordAuthor | MS/MS | - |
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