Dermal fibroblast expression of stromal cell-derived factor-1 (SDF-1) promotes epidermal keratinocyte proliferation in normal and diseased skin
DC Field | Value | Language |
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dc.contributor.author | Quan, Chunji | - |
dc.contributor.author | Cho, Moon Kyun | - |
dc.contributor.author | Shao, Yuan | - |
dc.contributor.author | Mianecki, Laurel E. | - |
dc.contributor.author | Liao, Eric | - |
dc.contributor.author | Perry, Daniel | - |
dc.contributor.author | Quan, Taihao | - |
dc.date.accessioned | 2021-08-11T19:25:03Z | - |
dc.date.available | 2021-08-11T19:25:03Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1674-800X | - |
dc.identifier.issn | 1674-8018 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10097 | - |
dc.description.abstract | Stromal cells provide a crucial microenvironment for overlying epithelium. Here we investigated the expression and function of a stromal cell-specific protein, stromal cell-derived factor-1 (SDF-1), in normal human skin and in the tissues of diseased skin. Immunohistology and laser capture microdissection (LCM)-coupled quantitative real-time RT-PCR revealed that SDF-1 is constitutively and predominantly expressed in dermal stromal cells in normal human skin in vivo. To our surprise, an extremely high level of SDF-1 transcription was observed in the dermis of normal human skin in vivo, evidenced by much higher mRNA expression level than type I collagen, the most abundant and highly expressed protein in human skin. SDF-1 was also upregulated in the tissues of many human skin disorders including psoriasis, basal cell carcinoma (BCC), and squamous cell carcinoma (SCC). Double immunostaining for SDF-1 and HSP47 (heat shock protein 47), a marker of fibroblasts, revealed that fibroblasts were the major source of stroma-cell-derived SDF-1 in both normal and diseased skin. Functionally, SDF-1 activates the ERK (extracellular-signal-regulated kinases) pathway and functions as a mitogen to stimulate epidermal keratinocyte proliferation. Both overexpression of SDF-1 in dermal fibroblasts and treatment with rhSDF-1 to the skin equivalent cultures significantly increased the number of keratinocyte layers and epidermal thickness. Conversely, the stimulative function of SDF-1 on keratinocyte proliferation was nearly completely eliminated by interfering with CXCR4, a specific receptor of SDF-1, or by knock-down of SDF-1 in fibroblasts. Our data reveal that extremely high levels of SDF-1 provide a crucial microenvironment for epidermal keratinocyte proliferation in both physiologic and pathologic skin conditions. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer Verlag | - |
dc.title | Dermal fibroblast expression of stromal cell-derived factor-1 (SDF-1) promotes epidermal keratinocyte proliferation in normal and diseased skin | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1007/s13238-015-0198-5 | - |
dc.identifier.scopusid | 2-s2.0-84948383380 | - |
dc.identifier.wosid | 000365514600005 | - |
dc.identifier.bibliographicCitation | Protein & Cell, v.6, no.12, pp 890 - 903 | - |
dc.citation.title | Protein & Cell | - |
dc.citation.volume | 6 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 890 | - |
dc.citation.endPage | 903 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | AGED HUMAN SKIN | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASE-1 | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | CXCR4 | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | SDF-1 | - |
dc.subject.keywordAuthor | dermal fibroblast | - |
dc.subject.keywordAuthor | keratinocyte | - |
dc.subject.keywordAuthor | proliferation | - |
dc.subject.keywordAuthor | skin cancer | - |
dc.subject.keywordAuthor | psoriasis | - |
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