Antimicrobial photodynamic therapy using chlorin e6 with halogen light for acne bacteria-induced inflammation
DC Field | Value | Language |
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dc.contributor.author | Jeon, Yu-Mi | - |
dc.contributor.author | Lee, Hwan-Suk | - |
dc.contributor.author | Jeong, Dongjun | - |
dc.contributor.author | Oh, Hae-Keun | - |
dc.contributor.author | Ra, Kyu-Hwan | - |
dc.contributor.author | Lee, Mi-Young | - |
dc.date.accessioned | 2021-08-11T20:26:43Z | - |
dc.date.available | 2021-08-11T20:26:43Z | - |
dc.date.issued | 2015-03-01 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.issn | 1879-0631 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10796 | - |
dc.description.abstract | Aims: The present study was designed to evaluate the therapeutic potential of antimicrobial photodynamic therapy (PDT) using chlorin e6 with halogen light against acne bacteria-induced inflammation. Main methods: Highly purified chlorin e6 (Ce6), as a second generation photosensitizer, was synthesized from Spirulina chlorophyll. To evaluate the antimicrobial property of Ce6-mediated PDT with halogen light, the broth microdilution method and two-color fluorescence assay were used. The free radicals generated upon irradiating Ce6 with halogen light were measured using 2,7-dichlorofluorescin diacetate. Propionibacterium acnes was intradermally injected into the left ear of the ICR mice, and the anti-inflammatory effect of Ce6-mediated PDT with halogen light was measured by the histological examination. The expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) as well as pro-inflammatory cytokines were also measured by Western blotting. Key findings: Chlorin e6-mediated PDT with halogen light (30,000 lx) inactivated various skin bacteria, including P. acnes in a dose-dependent manner. The MIC99 value against P. acnes (KCTC3314) of Ce6 with light was >0.49 mu g/ml, whereas the MIC99 for Ce6 alone was >31.25 mu g/ml. Ce6-mediated PDT suppressed the expression of P. acnes-induced pro-inflammatory cytokines and iNOS, but not COX-2 in a mouse model. Significance: This study showed a remarkable therapeutic effect of chlorin e6-mediated PDT with halogen light against P. acnes-induced inflammation. Our results suggest for the first time the potential of Ce6-mediated PDT with halogen light as a more effective and safer alternative treatment to antibiotic therapy against pathogenic infections of the skin. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Antimicrobial photodynamic therapy using chlorin e6 with halogen light for acne bacteria-induced inflammation | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.lfs.2014.12.029 | - |
dc.identifier.scopusid | 2-s2.0-84922469466 | - |
dc.identifier.wosid | 000352118100008 | - |
dc.identifier.bibliographicCitation | Life Sciences, v.124, pp 56 - 63 | - |
dc.citation.title | Life Sciences | - |
dc.citation.volume | 124 | - |
dc.citation.startPage | 56 | - |
dc.citation.endPage | 63 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | PROPIONIBACTERIUM-ACNES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | VULGARIS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | PHOTOSENSITIZER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | FLUORESCEIN | - |
dc.subject.keywordPlus | EFFICACY | - |
dc.subject.keywordAuthor | Chlorin e6 | - |
dc.subject.keywordAuthor | Halogen light | - |
dc.subject.keywordAuthor | Photodynamic therapy | - |
dc.subject.keywordAuthor | Anti-microbial effect | - |
dc.subject.keywordAuthor | Anti-inflammatory effect | - |
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