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Antimicrobial photodynamic therapy using chlorin e6 with halogen light for acne bacteria-induced inflammation

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dc.contributor.authorJeon, Yu-Mi-
dc.contributor.authorLee, Hwan-Suk-
dc.contributor.authorJeong, Dongjun-
dc.contributor.authorOh, Hae-Keun-
dc.contributor.authorRa, Kyu-Hwan-
dc.contributor.authorLee, Mi-Young-
dc.date.accessioned2021-08-11T20:26:43Z-
dc.date.available2021-08-11T20:26:43Z-
dc.date.issued2015-03-01-
dc.identifier.issn0024-3205-
dc.identifier.issn1879-0631-
dc.identifier.urihttps://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10796-
dc.description.abstractAims: The present study was designed to evaluate the therapeutic potential of antimicrobial photodynamic therapy (PDT) using chlorin e6 with halogen light against acne bacteria-induced inflammation. Main methods: Highly purified chlorin e6 (Ce6), as a second generation photosensitizer, was synthesized from Spirulina chlorophyll. To evaluate the antimicrobial property of Ce6-mediated PDT with halogen light, the broth microdilution method and two-color fluorescence assay were used. The free radicals generated upon irradiating Ce6 with halogen light were measured using 2,7-dichlorofluorescin diacetate. Propionibacterium acnes was intradermally injected into the left ear of the ICR mice, and the anti-inflammatory effect of Ce6-mediated PDT with halogen light was measured by the histological examination. The expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) as well as pro-inflammatory cytokines were also measured by Western blotting. Key findings: Chlorin e6-mediated PDT with halogen light (30,000 lx) inactivated various skin bacteria, including P. acnes in a dose-dependent manner. The MIC99 value against P. acnes (KCTC3314) of Ce6 with light was >0.49 mu g/ml, whereas the MIC99 for Ce6 alone was >31.25 mu g/ml. Ce6-mediated PDT suppressed the expression of P. acnes-induced pro-inflammatory cytokines and iNOS, but not COX-2 in a mouse model. Significance: This study showed a remarkable therapeutic effect of chlorin e6-mediated PDT with halogen light against P. acnes-induced inflammation. Our results suggest for the first time the potential of Ce6-mediated PDT with halogen light as a more effective and safer alternative treatment to antibiotic therapy against pathogenic infections of the skin. (C) 2015 Elsevier Inc. All rights reserved.-
dc.format.extent8-
dc.language영어-
dc.language.isoENG-
dc.publisherElsevier BV-
dc.titleAntimicrobial photodynamic therapy using chlorin e6 with halogen light for acne bacteria-induced inflammation-
dc.typeArticle-
dc.publisher.location영국-
dc.identifier.doi10.1016/j.lfs.2014.12.029-
dc.identifier.scopusid2-s2.0-84922469466-
dc.identifier.wosid000352118100008-
dc.identifier.bibliographicCitationLife Sciences, v.124, pp 56 - 63-
dc.citation.titleLife Sciences-
dc.citation.volume124-
dc.citation.startPage56-
dc.citation.endPage63-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaResearch & Experimental Medicine-
dc.relation.journalResearchAreaPharmacology & Pharmacy-
dc.relation.journalWebOfScienceCategoryMedicine, Research & Experimental-
dc.relation.journalWebOfScienceCategoryPharmacology & Pharmacy-
dc.subject.keywordPlusPROPIONIBACTERIUM-ACNES-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusANTIOXIDANT ACTIVITY-
dc.subject.keywordPlusVULGARIS-
dc.subject.keywordPlusASSAY-
dc.subject.keywordPlusACID-
dc.subject.keywordPlusPHOTOSENSITIZER-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusFLUORESCEIN-
dc.subject.keywordPlusEFFICACY-
dc.subject.keywordAuthorChlorin e6-
dc.subject.keywordAuthorHalogen light-
dc.subject.keywordAuthorPhotodynamic therapy-
dc.subject.keywordAuthorAnti-microbial effect-
dc.subject.keywordAuthorAnti-inflammatory effect-
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