Genome Wide Identification and Expression Profile in Epithelial Cells Exposed to TiO2 Particles
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Tae-Hoon | - |
dc.contributor.author | Shin, Seung-Woo | - |
dc.contributor.author | Park, Jong-Sook | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.date.accessioned | 2021-08-11T20:44:10Z | - |
dc.date.available | 2021-08-11T20:44:10Z | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1520-4081 | - |
dc.identifier.issn | 1522-7278 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10846 | - |
dc.description.abstract | Environmental particles are believed to provoke airway inflammation in susceptible individuals by stimulating epithelial cells to release mediators that exacerbate lung diseases. Here, we sought to identify genes expressed throughout the genome by epithelial cells stimulated with TiO2 particles. A human bronchial epithelial cell line, BEAS-2B, was stimulated with or without 40 mu g TiO2 for 2 h. RNA was purified from cells and subjected to microarray analysis. Genes exhibiting more than a twofold change in RNA expression were selected. Candidate genes were then analyzed using bioinformatics tools, including pathway, ontology, and network analyses. ITGAV mRNA expression levels were measured in BEAS-2B cells using real-time polymerase chain reaction. Among 37,803 genes, 92 genes displayed more than a twofold change in mRNA levels according to the microarray analysis; 87 genes were upregulated while five genes were downregulated. The 92 genes were classified based on functional annotation using a protein information resource database search for biological processes and a pathway search using the KEGG pathway database. These genes are related to macromolecule biosynthesis, metabolic processes and, in particular, RNA metabolism. When genes with more than a threefold change were analyzed, KIF11, ITGAV, SEMA3C, IBTK, and DEK were selected as candidate genes induced by TiO2-stimulated BEAS-2B cells. To validate these results, BEAS-2B cells stimulated with 40 mu g TiO2 expressed threefold higher ITGAV mRNA levels compared to those without TiO2 particle stimulation. We conclude that KIF11, ITGAV, SEMA3C, IBTK, and DEK are candidate genes expressed by epithelial cells when stimulated with TiO2 particles. (c) 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 293-300, 2015. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Genome Wide Identification and Expression Profile in Epithelial Cells Exposed to TiO2 Particles | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/tox.21906 | - |
dc.identifier.scopusid | 2-s2.0-84921728871 | - |
dc.identifier.wosid | 000349178300004 | - |
dc.identifier.bibliographicCitation | Environmental Toxicology, v.30, no.3, pp 293 - 300 | - |
dc.citation.title | Environmental Toxicology | - |
dc.citation.volume | 30 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 293 | - |
dc.citation.endPage | 300 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalResearchArea | Water Resources | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Water Resources | - |
dc.subject.keywordPlus | PARTICULATE AIR-POLLUTION | - |
dc.subject.keywordPlus | DAILY MORTALITY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | ADMISSIONS | - |
dc.subject.keywordPlus | MATTER | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | gene | - |
dc.subject.keywordAuthor | gene expression profile | - |
dc.subject.keywordAuthor | ITGAV | - |
dc.subject.keywordAuthor | titanium dioxide | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.