Gene structure, cDNA characterization and RNAi-based functional analysis of a myeloid differentiation factor 88 homolog in Tenebrio molitor larvae exposed to Staphylococcus aureus infection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Patnaik, Bharat Bhusan | - |
dc.contributor.author | Patnaik, Hongray Howrelia | - |
dc.contributor.author | Seo, Gi Won | - |
dc.contributor.author | Jo, Yong Hun | - |
dc.contributor.author | Lee, Yong Seok | - |
dc.contributor.author | Lee, Bok Luel | - |
dc.contributor.author | Han, Yeon Soo | - |
dc.date.accessioned | 2021-08-11T22:25:30Z | - |
dc.date.available | 2021-08-11T22:25:30Z | - |
dc.date.issued | 2014-10 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.issn | 1879-0089 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/11843 | - |
dc.description.abstract | Myeloid differentiation factor 88 (MyD88), an intracellular adaptor protein involved in Toll/Toll-like receptor (TLR) signal processing, triggers activation of nuclear factor-kappaB (NF-kappa B) transcription factors. In the present study, we analyzed the gene structure and biological function of MyD88 in a coleopteran insect, Tenebrio molitor (TmMyD88). The TmMyD88 gene was 1380 bp in length and consisted of five exons and four introns. The 5'-flanking sequence revealed several putative transcription factor binding sites, such as STAT-4, AP-1, cJun, cfos, NF-1 and many heat shock factor binding elements. The cDNA contained a typical death domain, a conservative Toll-like interleukin-1 receptor (TIR) domain, and a C-terminal extension (CTE). The TmMyD88 TIR domain showed three significantly conserved motifs for interacting with the TIR domain of TLRs. TmMyD88 was grouped within the invertebrate cluster of the phylogenetic tree and shared 75% sequence identity with the TIR domain of Tribolium castaneum MyD88. Homology modeling of the TmMyD88 TIR domain revealed five parallel beta-strands surrounded by five alpha-helices that adopted loop conformations to function as an adaptor. TmMyD88 expression was upregulated 7.3- and 4.79-fold after 12 and 6 h, respectively, of challenge with Staphylococcus aureus and fungal beta-1,3 glucan. Silencing of the TmMyD88 transcript by RNA interference led to reduced resistance of the host to infection by S. aureus. These results indicate that TmMyD88 is required for survival against Staphylococcus infection. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Pergamon Press Ltd. | - |
dc.title | Gene structure, cDNA characterization and RNAi-based functional analysis of a myeloid differentiation factor 88 homolog in Tenebrio molitor larvae exposed to Staphylococcus aureus infection | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.dci.2014.04.009 | - |
dc.identifier.scopusid | 2-s2.0-84900552162 | - |
dc.identifier.wosid | 000340216600011 | - |
dc.identifier.bibliographicCitation | Developmental and Comparative Immunology, v.46, no.2, pp 208 - 221 | - |
dc.citation.title | Developmental and Comparative Immunology | - |
dc.citation.volume | 46 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 208 | - |
dc.citation.endPage | 221 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | RECEPTOR TIR DOMAIN | - |
dc.subject.keywordPlus | TOLL PATHWAY | - |
dc.subject.keywordPlus | PROTEOLYTIC CASCADE | - |
dc.subject.keywordPlus | DROSOPHILA MYD88 | - |
dc.subject.keywordPlus | ADAPTER PROTEIN | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | CPG-DNA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | Tenebrio molitor | - |
dc.subject.keywordAuthor | MyD88 | - |
dc.subject.keywordAuthor | Toll/IL-1 domain | - |
dc.subject.keywordAuthor | Immune challenge | - |
dc.subject.keywordAuthor | RNA interference | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.