Anti-arthritis effects of ( E)-2,4-bis(p-hydroxyphenyl)2-butenal are mediated by inhibition of the STAT3 pathway
DC Field | Value | Language |
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dc.contributor.author | Ban, Jung Ok | - |
dc.contributor.author | Kim, Dae Hwan | - |
dc.contributor.author | Lee, Hee Pom | - |
dc.contributor.author | Hwang, Chul Ju | - |
dc.contributor.author | Shim, Jung-Hyun | - |
dc.contributor.author | Kim, Dae Joong | - |
dc.contributor.author | Kim, Tae Myoung | - |
dc.contributor.author | Jeong, Heon-Sang | - |
dc.contributor.author | Nah, Seong Su | - |
dc.contributor.author | Chen, Hanyong | - |
dc.contributor.author | Dong, Zigang | - |
dc.contributor.author | Ham, Young Wan | - |
dc.contributor.author | Kim, Youngsoo | - |
dc.contributor.author | Han, Sang-Bae | - |
dc.contributor.author | Hong, Jin Tae | - |
dc.date.accessioned | 2021-08-11T22:46:04Z | - |
dc.date.available | 2021-08-11T22:46:04Z | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.issn | 1476-5381 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/12186 | - |
dc.description.abstract | Background and PurposeProducts of Maillard reactions between aminoacids and reducing sugars are known to have anti-inflammatory properties. Here we have assessed the anti-arthritis effects of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal and its possible mechanisms of action. Experimental ApproachWe used cultures of LPS-activated macrophages (RAW264.7 cells) and human synoviocytes from patients with rheumatoid arthritis for in vitro assays and the collagen-induced arthritis model in mice. NO generation, iNOS and COX2 expression, and NF-B/IKK and STAT3 activities were measured in vitro and in joint tissues of arthritic mice, along with clinical scores and histopathological assessments. Binding of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal to STAT3 was evaluated by a pull-down assay and its binding site was predicted using molecular docking studies with Autodock VINA. Key Results(E)-2,4-bis(p-hydroxyphenyl)-2-butenal (2.5-10 gmL(-1)) inhibited LPS-inducedNO generation, iNOS and COX2 expression, and NF-B/IKK and STAT3 activities in macrophage and human synoviocytes. This compound also suppressedcollagen-induced arthritic responses in mice by inhibiting expression of iNOS and COX2, and NF-B/IKK and STAT3 activities; it also reduced bone destruction and fibrosis in joint tissues. A pull-down assay showed that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal interfered with binding of ATP to STAT3. Docking studies suggested that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal bound to the DNA-binding interface of STAT3 possibly inhibiting ATP binding to STAT3 in an allosteric manner. Conclusions and Implications(E)-2,4-bis(p-hydroxyphenyl)-2-butenal exerted anti-inflammatory and anti-arthritic effects through inhibition of the NF-B/STAT3 pathway by direct binding to STAT3. This compound could be a useful agent for the treatment of arthritic disease. | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Wiley-Blackwell | - |
dc.title | Anti-arthritis effects of ( E)-2,4-bis(p-hydroxyphenyl)2-butenal are mediated by inhibition of the STAT3 pathway | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/bph.12619 | - |
dc.identifier.scopusid | 2-s2.0-84900530191 | - |
dc.identifier.wosid | 000335862300015 | - |
dc.identifier.bibliographicCitation | British Journal of Pharmacology, v.171, no.11, pp 2900 - 2912 | - |
dc.citation.title | British Journal of Pharmacology | - |
dc.citation.volume | 171 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2900 | - |
dc.citation.endPage | 2912 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | FIBROBLAST-LIKE SYNOVIOCYTES | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | REACTIVE OXYGEN | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordAuthor | (E)-2 | - |
dc.subject.keywordAuthor | 4-bis(p-hydroxyphenyl)-2-butenal | - |
dc.subject.keywordAuthor | STAT3 | - |
dc.subject.keywordAuthor | NF-B | - |
dc.subject.keywordAuthor | arthritis | - |
dc.subject.keywordAuthor | inflammation | - |
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