Decreased Cellular Levels of Palmitic Amide are Linked to 5-fluorouracil Resistance in Human Colon Cancer Cells
- Authors
- Lee, Jun Hwa; Kim, Seung Cheol; Kim, Kyung-Hee; Kim, Won Ki; Lee, Min Jun; Yoo, Byong Chul; Yeo, Seung-Gu
- Issue Date
- Mar-2014
- Publisher
- Thieme
- Keywords
- Palmitic amide; 5-fluorouracil; Colon cancer; Proteomics
- Citation
- Hepato-Gastroenterology, v.61, no.130, pp 343 - 348
- Pages
- 6
- Journal Title
- Hepato-Gastroenterology
- Volume
- 61
- Number
- 130
- Start Page
- 343
- End Page
- 348
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/12373
- DOI
- 10.5754/hge13979
- ISSN
- 0172-6390
- Abstract
- Background/Aims: The aim of this study was to investigate whether profiling metabolic compounds in human colon cancer cells with induced 5-florouracil resistance enables identification of predictive bio-markers for 5-florouracil resistance. Methodology: 5-florouracil resistant and parental cells were extracted using methanol/chloroform solution, and analyzed by MALDI-TOF. Principal components analysis and discriminant analysis was performed to select low-mass ions with strong discriminating power between 5-florouracil resistant and parental cells. The correlation between the intensities of low-mass ions and intrinsic 5-florouracil resistance in 11 colon cancer cells was analyzed using the Spearman rank coefficient. Results: Eleven low-mass ions had strong discriminating power between 5-florouracil-resistant and parental cells. Of these, the intensity of a low-mass ion with 256.29m/z was negatively correlated with intrinsic 5-florouracil resistance in 11 colon cancer cells (r = -0.6545, P = 0.0338). By searching the H+ adduct with 0.05 m/z tolerance in the Human Metabolome Database, a low-mass ion of 256.29 m/z was identified as palmitic amide. Interestingly, extracellular treatment with palmitic amide reduced 5-florouracil resistance and invasiveness in 5-florouracil-resistant cells. Conclusions: Palmitic amide showed potential not only as a predictor of 5-florouracil resistance, but also for reduction of 5-florouracil resistance in colon cancer cells.
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Collections - College of Medicine > Department of Radiation Oncology > 1. Journal Articles
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