Molecular and immunohistochemical characterization of granulin gene encoded in Pieris rapae granulovirus genome
DC Field | Value | Language |
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dc.contributor.author | Oh, Seunghan | - |
dc.contributor.author | Kim, Dong Hyun | - |
dc.contributor.author | Patnaik, Bharat Bhusan | - |
dc.contributor.author | Jo, Yong Hun | - |
dc.contributor.author | Noh, Mi Young | - |
dc.contributor.author | Lee, Hyo Jeong | - |
dc.contributor.author | Lee, Kwang Ho | - |
dc.contributor.author | Yoon, Kwang Ho | - |
dc.contributor.author | Kim, Wan-Jong | - |
dc.contributor.author | Noh, Ju Young | - |
dc.contributor.author | Jeong, Heon Cheon | - |
dc.contributor.author | Lee, Yong Seok | - |
dc.contributor.author | Zhang, Chuan-Xi | - |
dc.contributor.author | Kim, Iksoo | - |
dc.contributor.author | Han, Yeon Soo | - |
dc.date.accessioned | 2021-08-12T01:17:26Z | - |
dc.date.available | 2021-08-12T01:17:26Z | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 0022-2011 | - |
dc.identifier.issn | 1096-0805 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13739 | - |
dc.description.abstract | Pieris rapae granulovirus (PiraGV) is highly pathogenic to the cabbage butterfly (P. rapae), an important pest of cultivated cabbages and mustard crops. It therefore holds significant promise towards exploitation as a potent bio-control agent in the field controlling the pest population. Whole-genome elucidation of the Korean isolate of the granulovirus (PiraGV-K), reported the presence of a granulin gene corresponding to ORF 1 in its genome. Comprehensive studies towards functional characterization of the gene, established that it is composed of 744 nucleotides and encodes a peptide of 247 amino acid residues. It possessed significant homology with AoGV and ClanGV with 87% identity at amino acid level. Multiple alignment data suggests that the C-terminus region of the gene had three different conserved regions. Time-course studies conducted in PiraGV-K infected P. rapae larvae revealed a significant upsurge of the transcript (134-fold) at 4 days post infection followed by a significant decline at the most advanced stages of infection. Anti-PiraGV-K granulin antibody was produced and western blot conducted with the infected larvae further confirmed the induction pattern with a protein of 30 kDa. Immunofluorescent staining showed a granulin-specific signal in fat body and integument of the infected larvae. Granulin-specific signals were noticed 2 days post infection with the eventual systemic spread of infection to the associated tracheal matrix witnessed at 4 days post infection. Immunogold labeling and electron microscopic studies further proved the cytopathological effects as the presence of numerous membrane-bound vesicles with nucleocapsids and abruption of intercellular junctions in fat body and hypertrophied cells in the integument. (c) 2012 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | Molecular and immunohistochemical characterization of granulin gene encoded in Pieris rapae granulovirus genome | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.jip.2012.12.003 | - |
dc.identifier.scopusid | 2-s2.0-84873699284 | - |
dc.identifier.wosid | 000317372000002 | - |
dc.identifier.bibliographicCitation | Journal of Invertebrate Pathology, v.113, no.1, pp 7 - 17 | - |
dc.citation.title | Journal of Invertebrate Pathology | - |
dc.citation.volume | 113 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 7 | - |
dc.citation.endPage | 17 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | PHYLOGENETIC ANALYSIS | - |
dc.subject.keywordPlus | POLYHEDROSIS-VIRUS | - |
dc.subject.keywordPlus | SEQUENCE-ANALYSIS | - |
dc.subject.keywordPlus | CONSERVED GENES | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | LEPIDOPTERA | - |
dc.subject.keywordPlus | REGION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BACULOVIRUSES | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordAuthor | Pieris rapae granulovirus | - |
dc.subject.keywordAuthor | PiraGV-Korea | - |
dc.subject.keywordAuthor | granulin | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | immunohistochemistry | - |
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