Successful mouse hepatocyte culture with sandwich collagen gel formation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyun Jung | - |
dc.contributor.author | Choi, Dongho | - |
dc.date.accessioned | 2021-08-12T01:18:15Z | - |
dc.date.available | 2021-08-12T01:18:15Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 2233-7903 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13809 | - |
dc.description.abstract | Purpose: Primary mammalian hepatocytes largely retain their liver-specific functions when they are freshly derived from donors. However, long-term cultures of functional hepatocytes are difficult to establish. To increase the longevity and maintain the differentiated functions of hepatocytes in primary culture, cells can be cultured in a sandwich configuration of collagen. In such a configuration, hepatocytes can be cultured for longer periods compared with cultures on single layers of collagen. However, research regarding mouse hepatocytes in sandwich culture is lacking. Methods: Primary mouse hepatocytes were sandwiched between two layers of collagen to maintain the stability of their liver-specific functions. After gelation, 2 mL of hepatocyte culture medium was applied. Results: After 24 hours, 5, 10 days of culture, the collagen gel sandwich maintained the cellular border and numbers of bile canaliculi more efficiently than a single collagen coating in both high and low density culture dishes. Reverse transcription-polymerase chain reaction analysis of alpha-l-antitrypsin (AAT), hepatocyte nuclear factor 4 alpha (HNF4A), alphafetoprotein, albumin, tryptophan oxygenase (TO), the tyrosine aminotransferase gene, glucose-6-phosphatase, glyceraldehyde-3- phosphate dehydrogenase for mouse primary hepatocytes cultured on collagen coated dishes and collagen gels showed superior hepatocyte-related gene expression in cells grown using the collagen gel sandwich culture system. AAT, HNF4A, albumin, TO were found to be expressed in mouse hepatocytes cultured on collagen gels for 5 and 10 days. In contrast, mouse hepatocytes grown on collagen-coated dishes did not express these genes after 5 and 10 days of culture. Conclusion: The collagen gel sandwich method is suitable for primary culture system of adult mouse hepatocytes. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Successful mouse hepatocyte culture with sandwich collagen gel formation | - |
dc.type | Article | - |
dc.identifier.doi | 10.4174/jkss.2013.84.4.202 | - |
dc.identifier.scopusid | 2-s2.0-84876019607 | - |
dc.identifier.wosid | 000317022100002 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Surgical Society, v.84, no.4, pp 202 - 208 | - |
dc.citation.title | Journal of the Korean Surgical Society | - |
dc.citation.volume | 84 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 202 | - |
dc.citation.endPage | 208 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001752332 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | PRIMARY RAT HEPATOCYTES | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | BILIARY-EXCRETION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Collagen | - |
dc.subject.keywordAuthor | Culture | - |
dc.subject.keywordAuthor | Hepatocyte | - |
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