Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers

Abstract

An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy)32+-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy)32+-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 530 mu gmL1. The detection limit of IgG is 1.5 mu gmL(-1).