Inhibitory effect of nicardipine on rotenone-induced apoptosis in SH-SY5Y human neuroblastoma cells
DC Field | Value | Language |
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dc.contributor.author | Park, Hae Jeong | - |
dc.contributor.author | Kim, Hak-Jae | - |
dc.date.accessioned | 2021-08-12T01:18:51Z | - |
dc.date.available | 2021-08-12T01:18:51Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.issn | 1791-3004 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13863 | - |
dc.description.abstract | Previous studies have demonstrated that calcium channel blockers have protective effects on damaged brains. In the present study, the protective effect of the calcium channel blocker nicardipine against rotenone-induced apoptosis in SH-SY5Y human neuroblastoma cells was investigated, focusing on mitogen-activated protein kinases (MAPKs) and caspase (CASP)-mediated apoptotic events. Nicardipine was found to decrease rotenone-induced apoptosis through 4,6-diamidino-2-phenylindole staining and the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay. In addition, nicardipine was identified to inhibit rotenone-induced elevation of intracellular Ca2+ concentration measured using the Fluo-4 AM fluorescent dye. Rotenone increased phosphorylation of c-Jun NH2-terminal kinase (JNK) and p38 MAPK, whereas nicardipine blocked these increases. Nicardipine also prevented clownregulation of B-cell CLL/lymphoma 2 and upregulation of Bc12-associated X protein by rotenone. Furthermore, nicardipine abrogated cleavage of CASP9 and 3 and poly (ADP-ribose) polymerase-1 by rotenone and CASP3 enzyme activity in rotenone-treated cells. These results indicate that nicardipine exerts a protective effect against rotenone-induced apoptosis in SH-SY5Y cells, inhibiting phosphorylation of JNK and p38 MAPK and activation of CASPs. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Spandidos Publications | - |
dc.title | Inhibitory effect of nicardipine on rotenone-induced apoptosis in SH-SY5Y human neuroblastoma cells | - |
dc.type | Article | - |
dc.publisher.location | 그리이스 | - |
dc.identifier.doi | 10.3892/mmr.2013.1260 | - |
dc.identifier.scopusid | 2-s2.0-84873138404 | - |
dc.identifier.wosid | 000315423600036 | - |
dc.identifier.bibliographicCitation | Molecular Medicine Reports, v.7, no.3, pp 941 - 946 | - |
dc.citation.title | Molecular Medicine Reports | - |
dc.citation.volume | 7 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 941 | - |
dc.citation.endPage | 946 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | BRAIN-INJURY | - |
dc.subject.keywordPlus | MAP KINASES | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | P38 | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordAuthor | nicardipine | - |
dc.subject.keywordAuthor | rotenone | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | caspase | - |
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