Mixtures of glyphosate and surfactant TN20 accelerate cell death via mitochondrial damage-induced apoptosis and necrosis
DC Field | Value | Language |
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dc.contributor.author | Kim, Young-hee | - |
dc.contributor.author | Hong, Jung-rak | - |
dc.contributor.author | Gil, Hyo-wook | - |
dc.contributor.author | Song, Ho-yeon | - |
dc.contributor.author | Hong, Sae-yong | - |
dc.date.accessioned | 2021-08-12T01:25:16Z | - |
dc.date.available | 2021-08-12T01:25:16Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13948 | - |
dc.description.abstract | Glyphosate, a common herbicide, is not toxic under normal exposure circumstances. However, this chemical, when combined with a surfactant, is cytotoxic. In this study, the mechanism of the additive effect of glyphosate and TN-20, a common surfactant in glyphosate herbicides, was investigated. After exposure of rat H9c2 cells to glyphosate and TN-20 mixtures, following assays were performed: flow cytometry to determine the proportion of cells that underwent apoptosis and necrosis; western blotting to determine expression of mitochondrial proteins (Bcl-2 and Bax); immunological methods to evaluate translocation of cytochrome C; luminometric measurements to determine activity of caspases 3/7 and 9; and tetramethyl rhodamine methyl ester assay to measure mitochondrial membrane potentials. Bcl-1 intensity decreased while Bax intensity increased with exposure to increasing TN-20 and/or glyphosate concentrations. Caspase activity increased and mitochondrial membrane potential decreased only when the cells were exposed to a mixture of both TN-20 and glyphosate, but not after exposure to either one of these compounds. The results support the possibility that mixtures of glyphosate and TN-20 aggravate mitochondrial damage and induce apoptosis and necrosis. Throughout this process, TN-20 seems to disrupt the integrity of the cellular barrier to glyphosate uptake, promoting glyphosate-mediated toxicity. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Mixtures of glyphosate and surfactant TN20 accelerate cell death via mitochondrial damage-induced apoptosis and necrosis | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.tiv.2012.09.021 | - |
dc.identifier.scopusid | 2-s2.0-84869446482 | - |
dc.identifier.wosid | 000314371600022 | - |
dc.identifier.bibliographicCitation | Toxicology in Vitro, v.27, no.1, pp 191 - 197 | - |
dc.citation.title | Toxicology in Vitro | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 191 | - |
dc.citation.endPage | 197 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | INTOXICATION | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordAuthor | Glyphosate | - |
dc.subject.keywordAuthor | Surfactant | - |
dc.subject.keywordAuthor | Mitochondrial damage | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Necrosis | - |
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