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Removal of Crude Oil by Microbial Consortium Isolated from Oil-Spilled Area in the Korean Western Coast
- Authors
- Cho, Su-Hee; Oh, Kye-Heon
- Issue Date
- Sep-2012
- Publisher
- Springer Verlag
- Keywords
- Crude oil removal; Microbial consortium; Oil-spilled coastal area; Tae-An
- Citation
- Bulletin of Environmental Contamination and Toxicology, v.89, no.3, pp 680 - 685
- Pages
- 6
- Journal Title
- Bulletin of Environmental Contamination and Toxicology
- Volume
- 89
- Number
- 3
- Start Page
- 680
- End Page
- 685
- ISSN
- 0007-4861
1432-0800
- Abstract
- The feasibility of using an indigenous microbial consortium for the removal of crude oil from an oil-spilled coastal area was explored with the ultimate aim of applying for bioremediation. Initially, we obtained the microbial consortium TK-2 that catalyzed the dispersion as well as the degradation of crude oil in supplemented sea water. GC and GC-MS were used to evaluate the removal patterns of crude oil during the incubation. The effective removal of crude oil by TK-2 occurred, and above 95 % of all aliphatic and aromatic compounds detected in this work was removed within 30 days of incubation. Two predominant crude oil-grown isolates derived from TK-2 revealed Gram-negative, rod-shaped cells. Both BIOLOG system and 16S rRNA sequencing were conducted to identify the strains, which were assigned to Arthrobacter sp. HK-2 and Pseudoalteromonas sp. HK-3, and registered in GenBank as [FJ477042] and [FJ477041].
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