Effect of freezing stress on the proteome expression of Antarctic green microalga
- Authors
- Choi, Kwang-Man; Lee, Mi-Young
- Issue Date
- 30-Jun-2012
- Publisher
- 대한독성 유전단백체 학회
- Keywords
- Freezing stress; Proteomics; Orthologues; Antioxidant enzyme; Antarctic green microalga
- Citation
- Molecular & Cellular Toxicology, v.8, no.2, pp 163 - 169
- Pages
- 7
- Journal Title
- Molecular & Cellular Toxicology
- Volume
- 8
- Number
- 2
- Start Page
- 163
- End Page
- 169
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15055
- DOI
- 10.1007/s13273-012-0020-x
- ISSN
- 1738-642X
2092-8467
- Abstract
- The effects of freezing stress on the protein expression profile and enzyme activity change in Antarctic green microalga were examined in this investigation. Antarctic green microalga was cultured at 4 degrees C for optimal growth, and the temperature was reduced near to 0 degrees C in order to apply freezing stress. The resultant differentially expressed proteins induced by freezing stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis (2-DE). In total, about 320 protein spots were detected on the 2-DE gel, and 17 proteins showed a greater than two-fold change in abundance; of these, 5 proteins were up-regulated, and 12 proteins were down-regulated. Moreover, total enzyme activities and isozyme patterns of several antioxidant enzymes, named catalase, superoxide dismutase and aldehyde dehydrogenase were changed upon applying freezing stress.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
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