PPAR gamma inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Yong Sung | - |
dc.contributor.author | Lillehoj, Erik P. | - |
dc.contributor.author | Kato, Kosuke | - |
dc.contributor.author | Park, Choon Sik | - |
dc.contributor.author | Kim, Kwang Chul | - |
dc.date.accessioned | 2021-08-12T03:27:20Z | - |
dc.date.available | 2021-08-12T03:27:20Z | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 1040-0605 | - |
dc.identifier.issn | 1522-1504 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15282 | - |
dc.description.abstract | Park YS, Lillehoj EP, Kato K, Park CS, Kim KC. PPAR gamma inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism. Am J Physiol Lung Cell Mol Physiol 302: L679-L687, 2012. First published January 20, 2012; doi: 10.1152/ajplung.00360.2011.-This study was conducted to examine the relationship between the peroxisome proliferator-associated receptor-gamma (PPAR gamma) and MUC1 mucin, two anti-inflammatory molecules expressed in the airways. Treatment of A549 lung epithelial cells or primary mouse tracheal surface epithelial (MTSE) cells with phorbol 12-myristate 13-acetate (PMA) increased the levels of tumor necrosis factor (TNF)-alpha in cell culture media compared with cells treated with vehicle alone. Over-expression of MUC1 in A549 cells decreased PMA-stimulated TNF-alpha levels, whereas deficiency of Muc1 expression in MTSE cells from Muc1 null mice increased PMA-induced TNF-alpha levels. Treatment of A549 or MTSE cells with the PPAR gamma agonist troglitazone (TGN) blocked the ability of PMA to stimulate TNF-alpha levels. However, the effect of TGN required the presence of MUC1/Muc1, since no differences in TNF-alpha levels were seen between PMA and PMA plus TGN in MUC1/Muc1-deficient cells. Similarly, whereas TGN decreased interleukin-8 (IL-8) levels in culture media of MUC1-expressing A549 cells treated with Pseudomonas aeruginosa strain K (PAK), no differences in IL-8 levels were seen between PAK and PAK plus TGN in MUC1-nonexpressing cells. EMSA confirmed the presence of a PPAR gamma-binding element in the MUC1 gene promoter. Finally, TGN treatment of A549 cells increased MUC1 promoter activity measured using a MUC1-luciferase reporter gene, augmented MUC1 mRNA levels by quantitative RT-PCR, and enhanced MUC1 protein expression by Western blot analysis. These combined data are consistent with the hypothesis that PPAR gamma stimulates MUC1/Muc1 expression, thereby blocking PMA/PAK-induced TNF-alpha/IL-8 production by airway epithelial cells. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Physiological Society | - |
dc.title | PPAR gamma inhibits airway epithelial cell inflammatory response through a MUC1-dependent mechanism | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1152/ajplung.00360.2011 | - |
dc.identifier.scopusid | 2-s2.0-84859473933 | - |
dc.identifier.wosid | 000302340200005 | - |
dc.identifier.bibliographicCitation | American Journal of Physiology - Lung Cellular and Molecular Physiology, v.302, no.7, pp 1679 - 1687 | - |
dc.citation.title | American Journal of Physiology - Lung Cellular and Molecular Physiology | - |
dc.citation.volume | 302 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1679 | - |
dc.citation.endPage | 1687 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalResearchArea | Respiratory System | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.subject.keywordPlus | ACTIVATED-RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | MUC1 TRANSCRIPTION | - |
dc.subject.keywordPlus | ALLERGIC INFLAMMATION | - |
dc.subject.keywordPlus | NEGATIVE REGULATOR | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | LUNG-DISEASE | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordAuthor | lung | - |
dc.subject.keywordAuthor | peroxisome proliferator-associated receptor-gamma | - |
dc.subject.keywordAuthor | PMA | - |
dc.subject.keywordAuthor | Pseudomonas aeruginosa | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.