Human Placenta Extract Could Promote Proliferating Cell Nuclear Antigen Expression during Liver Regeneration Induced by Partial Hepatectomy in RatsHuman Placenta Extract Could Promote Proliferating Cell Nuclear Antigen Expression during Liver Regeneration Induced by Partial Hepatectomy in Rats
- Other Titles
- Human Placenta Extract Could Promote Proliferating Cell Nuclear Antigen Expression during Liver Regeneration Induced by Partial Hepatectomy in Rats
- Authors
- 김지현; 한규범; 최용수; 이영준; 윤광호; 한만덕; 김완종
- Issue Date
- 2012
- Publisher
- 한국현미경학회
- Keywords
- Human placenta extract; Liver regeneration; Partial hepatectomy; PCNA
- Citation
- 한국현미경학회지, v.42, no.3, pp 115 - 123
- Pages
- 9
- Journal Title
- 한국현미경학회지
- Volume
- 42
- Number
- 3
- Start Page
- 115
- End Page
- 123
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15653
- ISSN
- 2287-5123
2287-4445
- Abstract
- Human placenta extract (hPE) has therapeutic potential against certain diseases such as burn injury, liver cirrhosis and chronic wound through stimulating tissue repair processes. However, the effects of hPE on liver regeneration in animals are unknown.
This study investigated the effect of hPE on the expression of proliferating cell nuclear antigen (PCNA) during liver regeneration induced by partial hepatectomy (PH) in rats.
The activities of AST, ALT and ALP increased during a few days after PH. A high level of ALP was particularly seen at day 3 in the control group. All the levels of experimental groups were normalized by day 5 after PH. On immunohistochemistry, the expression of PCNA increased at the early days, showed a peak at day 3 after PH. The PCNA staining was more obvious in the experimental group over the whole period. By western blotting,PCNA seemed to be more strongly expressed in the hPE injected group in the early stage and fell to almost undetectable levels at day 7. On immunocytochemical observations, the number of PCNA-gold particles in the nuclei at day 1 of the hPE treated groups was more than those of the untreated groups. The results suggest that hPE could accelerate liver regeneration induced by PH involving the expression of PCNA in rats.
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Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
- College of Natural Sciences > Department of Life Science and Biotechnology > 1. Journal Articles
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