Relationship between Group-Specific Component Protein and the Development of Asthma
DC Field | Value | Language |
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dc.contributor.author | Lee, Shin-Hwa | - |
dc.contributor.author | Kim, Kyung-Hun | - |
dc.contributor.author | Kim, Jin-Moo | - |
dc.contributor.author | Yoon, Sang-Hyuk | - |
dc.contributor.author | Kim, Tae Hoon | - |
dc.contributor.author | Park, Sung-Woo | - |
dc.contributor.author | Park, Jong-Sook | - |
dc.contributor.author | Uh, Soo-Taek | - |
dc.contributor.author | Lee, Ho Sung | - |
dc.contributor.author | Kim, Yong Hoon | - |
dc.contributor.author | Kang, Jin Hyun | - |
dc.contributor.author | Chung, Il Yup | - |
dc.contributor.author | Paik, Young Ki | - |
dc.contributor.author | Rhim, Taiyoun | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.date.accessioned | 2021-08-12T04:48:17Z | - |
dc.date.available | 2021-08-12T04:48:17Z | - |
dc.date.issued | 2011-09-01 | - |
dc.identifier.issn | 1073-449X | - |
dc.identifier.issn | 1535-4970 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16211 | - |
dc.description.abstract | Rationale: Airway inflammation and remodeling during asthma are attributed to the altered expression of biologically relevant proteins. Objectives: To search for asthma-specific proteins in bronchoalveolar lavage fluid (BAL) from individuals with asthma and to validate the identified proteins in an experimental model of asthma. Methods: Liquid chromatography-tandem mass spectrometry was performed to identify proteins in BAL fluid found by two-dimensional electrophoresis (2DE) to be differentially expressed in subjects with asthma versus control subjects. Group-specific component (Gc) and mRNA levels were measured using an ELISA, Western blots, and PCR. A neutralization study using an antibody against Gc protein was performed in an experimental asthma model. Measurements and Main Results: Based on 2DE, 15 proteins were significantly up-regulated or down-regulated in eight subjects with asthma compared with eight control subjects. The protein levels of Gc, hemopexin, and haptoglobin-beta were increased, whereas the alpha(1)-antitrypsin and glutathione S-transferase levels were decreased in subjects with asthma. The Gc concentration in BAL fluid was significantly elevated in 67 subjects with asthma compared with that in 22 control subjects (P < 0.009). The Gc was significantly correlated with the neutrophil percentage in BAL fluid of subjects with asthma (P = 0.001). Gc mRNA and protein levels were higher in ovalbumin-sensitized/challenged asthma mice than in sham-treated mice. Gc protein were expressed on alveolar macrophages and on epithelial cells. Treatment with an anti-Gc antibody dose-dependently reduced the ovalbumin sensitization/challenge-induced enhancement of airway hyperreactivity, airway inflammation, goblet cell hyperplasia, and levels of eotaxin, interleukin-4, -5, and -13, and interferon-g. Conclusions: Gc may be involved in the development of asthma, and the neutralization of Gc protein could be a therapeutic strategy for asthma. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Thoracic Society | - |
dc.title | Relationship between Group-Specific Component Protein and the Development of Asthma | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1164/rccm.201006-0951OC | - |
dc.identifier.scopusid | 2-s2.0-80052464259 | - |
dc.identifier.wosid | 000294478200013 | - |
dc.identifier.bibliographicCitation | American Journal of Respiratory and Critical Care Medicine, v.184, no.5, pp 528 - 536 | - |
dc.citation.title | American Journal of Respiratory and Critical Care Medicine | - |
dc.citation.volume | 184 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 528 | - |
dc.citation.endPage | 536 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalResearchArea | Respiratory System | - |
dc.relation.journalWebOfScienceCategory | Critical Care Medicine | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.subject.keywordPlus | D-BINDING-PROTEIN | - |
dc.subject.keywordPlus | BRONCHOALVEOLAR LAVAGE FLUID | - |
dc.subject.keywordPlus | VITAMIN-D | - |
dc.subject.keywordPlus | PROTEOMIC ANALYSIS | - |
dc.subject.keywordPlus | GC-GLOBULIN | - |
dc.subject.keywordPlus | NEUTROPHIL RECRUITMENT | - |
dc.subject.keywordPlus | POTENTIAL MECHANISM | - |
dc.subject.keywordPlus | ALLERGIC-ASTHMA | - |
dc.subject.keywordPlus | LUNG INJURY | - |
dc.subject.keywordPlus | MOUSE MODEL | - |
dc.subject.keywordAuthor | asthma | - |
dc.subject.keywordAuthor | BAL | - |
dc.subject.keywordAuthor | Gc | - |
dc.subject.keywordAuthor | proteomics | - |
dc.subject.keywordAuthor | 2D electrophoresis | - |
dc.subject.keywordAuthor | therapeutics | - |
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