Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model
DC Field | Value | Language |
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dc.contributor.author | Kwon, Soon Won | - |
dc.contributor.author | Sohn, Eun Jeong | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Jeong, Hoon Jae | - |
dc.contributor.author | Kim, Mi Jin | - |
dc.contributor.author | Ahn, Eun Hee | - |
dc.contributor.author | Kim, Young Nam | - |
dc.contributor.author | Dutta, Suman | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Hwang, Hyun Sook | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2021-08-12T05:25:38Z | - |
dc.date.available | 2021-08-12T05:25:38Z | - |
dc.date.issued | 2011-07-29 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16336 | - |
dc.description.abstract | Heme oxygenase-1 (HO-1), which catalyzes the degradation of free heme to biliverdin, carbon monoxide (CO), and free iron (Fe2+), is up-regulated by several cellular stress and cell injuries, including inflammation, ischemia and hypoxia. In this study, we examined whether fusion of HO-1 with PEP-1, a protein transduction domain that is able to deliver exogenous molecules to living cells or tissues, would facilitate HO-1 delivery to target cells and tissues, and thereby effectively exert a therapeutically useful response against inflammation. Western blot analysis demonstrated that PEP-1-HO-1 fusion proteins were transduced into Raw 264.7 cells in time- and dose-dependent manners, and were stably maintained in the cells for about 60 h. In addition, fluorescence analysis revealed that only PEP-1-HO-1 fusion proteins were significantly transduced into the cytoplasm of cells, while HO-1 proteins failed to be transduced. In lipopolysaccharide (LPS)-stimulated Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse edema model, transduced PEP-1-HO-1 fusion proteins effectively inhibited the overexpression of pro-inflammatory mediators and cytokines. Also, histological analysis demonstrated that PEP-1-HO-1 remarkably suppressed ear edema. The results suggest that the PEP-1-HO-1 fusion protein can be used as a therapeutic molecule against reactive oxygen species-related inflammatory diseases. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.06.147 | - |
dc.identifier.scopusid | 2-s2.0-79960843148 | - |
dc.identifier.wosid | 000293809900022 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, v.411, no.2, pp 354 - 359 | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.volume | 411 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 354 | - |
dc.citation.endPage | 359 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | VIVO PROTEIN TRANSDUCTION | - |
dc.subject.keywordPlus | THERAPEUTIC APPLICATIONS | - |
dc.subject.keywordPlus | CARBON-MONOXIDE | - |
dc.subject.keywordPlus | HEME OXYGENASE | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordAuthor | PEP-1-HO-1 | - |
dc.subject.keywordAuthor | Protein transduction domain | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | TPA | - |
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