Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models
DC Field | Value | Language |
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dc.contributor.author | Kim, So Young | - |
dc.contributor.author | Jeong, Hoon Jae | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Kim, Mi Jin | - |
dc.contributor.author | An, Jae Jin | - |
dc.contributor.author | Sohn, Eun Jeong | - |
dc.contributor.author | Kang, Hye Won | - |
dc.contributor.author | Shin, Min Jea | - |
dc.contributor.author | Ahn, Eun Hee | - |
dc.contributor.author | Kwon, Soon Won | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Cho, Sung-Woo | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2021-08-12T05:25:57Z | - |
dc.date.available | 2021-08-12T05:25:57Z | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0171-2985 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16363 | - |
dc.description.abstract | FK506 binding protein 12 (FK506BP) is an immunophilin that acts as a receptor for the immunosuppressant drug FK506. Although the precise action of FK506BP remains unclear, it has emerged as a potential drug target for several inflammatory diseases. This study investigated the protective effects of FK506BP on inflammation in vitro and in vivo using protein transduction. A cell-permeable expression vector PEP-1-FK506BP was constructed. Lipopolysaccharide (LPS)- or 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated Raw 264.7 cells and ICR mice were treated with PEP-1-FK506BP. The expression of inflammatory response enzymes and cytokines was analyzed by Western blot, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and electrophoretic mobility shift assay. PEP-1-FK506BP efficiently transduced into Raw 264.7 cells and markedly inhibited the expression levels of cyclooxygenase-2 as well as pro-inflammatory cytokines. Furthermore, transduced PEP-1-FK506BP significantly reduced activation of nuclear factor-kappa B (NF-kappa B) and phosphorylation of p38 mitogen-activated protein kinase (MAPK) in the cells, whereas PEP-1-FK506BP reduced phosphorylation of p38 and extracellular signal-regulated kinase (ERK) in the animal models. These results indicate that PEP-1-FK506BP inhibits inflammatory response cytokines and enzymes by blocking NF-kappa B and MAPK including the phosphorylation of p38 and/or ERK MAPK in vitro and in vivo, suggesting that PEP-1-FK506BP may be a therapeutic agent against inflammatory skin diseases. (C) 2010 Elsevier GmbH. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cell and mouse models | - |
dc.type | Article | - |
dc.publisher.location | 독일 | - |
dc.identifier.doi | 10.1016/j.imbio.2010.12.008 | - |
dc.identifier.scopusid | 2-s2.0-79957602694 | - |
dc.identifier.wosid | 000293667300003 | - |
dc.identifier.bibliographicCitation | Immunobiology, v.216, no.7, pp 771 - 781 | - |
dc.citation.title | Immunobiology | - |
dc.citation.volume | 216 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 771 | - |
dc.citation.endPage | 781 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PHORBOL ESTER 12-O-TETRADECANOYLPHORBOL-13-ACETATE | - |
dc.subject.keywordPlus | PROTEIN EFFICIENTLY PROTECTS | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | SKIN INFLAMMATION | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | CIS/TRANS ISOMERASES | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | MURINE MACROPHAGES | - |
dc.subject.keywordPlus | ISCHEMIC INSULT | - |
dc.subject.keywordAuthor | Cytokines | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | PEP-1-FK506BP | - |
dc.subject.keywordAuthor | Protein therapy | - |
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