Altering sexual reproductive mode by interspecific exchange of MAT loci
DC Field | Value | Language |
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dc.contributor.author | Lu, Shun-Wen | - |
dc.contributor.author | Yun, Sung-Hwan | - |
dc.contributor.author | Lee, Theresa | - |
dc.contributor.author | Turgeon, B. Gillian | - |
dc.date.accessioned | 2021-08-12T05:26:15Z | - |
dc.date.available | 2021-08-12T05:26:15Z | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 1087-1845 | - |
dc.identifier.issn | 1096-0937 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16380 | - |
dc.description.abstract | Sexual fungi can be self-sterile (heterothallic, requiring genetically distinct partners) or self-fertile (homothallic, no partner required). In most ascomycetes, a single mating type locus (MAT) controls the ability to reproduce sexually. In the genus Cochliobolus, all heterothallic species have either MAT1-1 or MAT1-2 (but never both) in different individuals whereas all homothallic species carry both MAT1-1 and MAT1-2 in the same nucleus of an individual. It has been demonstrated, previously, that a MAT gene from homothallic Cochliobolus luttrellii can confer self-mating ability on a mat-deleted strain of its heterothallic relative, Cochliobolus heterostrophus. In this reciprocal study, we expressed, separately, the heterothallic C. heterostrophus MAT1-1-1 and MAT1-2-1 genes in a mat-deleted homothallic C. luttrellii strain and asked if this converts homothallic C. luttrellii to heterothallism. We report that: (1) A C. luttrellii transgenic strain carrying C. heterostrophus MAT1-1-1 and a C. luttrellii transgenic strain carrying C. heterostrophus MAT1-2-1 can mate in a heterothallic manner and the fertility of the cross is similar to that of a wild type C. luttrellii self. Full tetrads are always found. (2) A C. luttrellii transgenic strain carrying C. heterostrophus MAT1-1-1 can mate with the parental wild type C. luttrellii MAT1-1;MAT1-2 strain, indicating the latter is able to outcross, a result which was expected but has not been demonstrated previously. (3) A C. luttrellii transgenic strain carrying C. heterostrophus MAT1-2-1 cannot mate with the parental wild type C. luttrellii MAT1-1;MAT1-2 strain, indicating outcrossing specificity. (4) Each transgenic C. luttrellii strain, carrying only a single C. heterostrophus MAT gene, is able to self, although all pseudothecia produced are smaller than those of wild type and fertility is low (about 4-15% of the number of wild type asci). These data support the argument that in Cochliobolus spp., the primary determinant of reproductive mode is MAT itself, and that a heterothallic strain can be made homothallic or a homothallic strain can be made heterothallic by exchange of MAT genes. The selfing ability of transgenic C luttrellii strains also suggests that both MAT1-1-1 and MAT1-2-1 genes of C. heterostrophus carry equivalent transcription regulatory activities, each capable of promoting sexual development when alone, in a suitable genetic background. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | Altering sexual reproductive mode by interspecific exchange of MAT loci | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.fgb.2011.04.006 | - |
dc.identifier.scopusid | 2-s2.0-79956106882 | - |
dc.identifier.wosid | 000291141200008 | - |
dc.identifier.bibliographicCitation | Fungal Genetics and Biology, v.48, no.7, pp 714 - 724 | - |
dc.citation.title | Fungal Genetics and Biology | - |
dc.citation.volume | 48 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 714 | - |
dc.citation.endPage | 724 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Mycology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Mycology | - |
dc.subject.keywordPlus | COCHLIOBOLUS-HETEROSTROPHUS | - |
dc.subject.keywordPlus | GIBBERELLA-ZEAE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ASPERGILLUS-NIDULANS | - |
dc.subject.keywordPlus | SORDARIA-MACROSPORA | - |
dc.subject.keywordPlus | HOMOTHALLIC FUNGUS | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | ASCOMYCETE | - |
dc.subject.keywordPlus | NEUROSPORA | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordAuthor | Reproductive mode | - |
dc.subject.keywordAuthor | Heterothallism | - |
dc.subject.keywordAuthor | Homothallism | - |
dc.subject.keywordAuthor | Mating type genes | - |
dc.subject.keywordAuthor | Cochliobolus heterostrophus | - |
dc.subject.keywordAuthor | Cochliobolus luttrellii | - |
dc.subject.keywordAuthor | Filamentous ascomycetes | - |
dc.subject.keywordAuthor | Sexual reproduction | - |
dc.subject.keywordAuthor | Homothallic | - |
dc.subject.keywordAuthor | Heterothallic | - |
dc.subject.keywordAuthor | Mating | - |
dc.subject.keywordAuthor | MAT | - |
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