Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT
DC Field | Value | Language |
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dc.contributor.author | Kim, Yul Ho | - |
dc.contributor.author | Lee, Yu Young | - |
dc.contributor.author | Kim, Yong Ho | - |
dc.contributor.author | Choi, Man Soo | - |
dc.contributor.author | Jeong, Kwang Ho | - |
dc.contributor.author | Lee, Seuk Ki | - |
dc.contributor.author | Seo, Min Jung | - |
dc.contributor.author | Yun, Hong Tai | - |
dc.contributor.author | Lee, Choon Ki | - |
dc.contributor.author | Kim, Wook Han | - |
dc.contributor.author | Lee, Sang Chul | - |
dc.contributor.author | Park, Soon Ki | - |
dc.contributor.author | Park, Hyang Mi | - |
dc.date.accessioned | 2021-08-12T05:47:33Z | - |
dc.date.available | 2021-08-12T05:47:33Z | - |
dc.date.issued | 2011-01-26 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16753 | - |
dc.description.abstract | Tocochromanols are potent lipid-soluble antioxidants and essential nutrients for human health. Genetic engineering techniques were used to develop soybeans with enhanced vitamin E levels, including tocotrienols, which are not found in soybean. The gene encoding rice homogentisate geranylgeranyl transferase (HGGT) was overexpressed in soybeans using seed-specific and constitutive promoters. The association between abundance of vitamin E isomers and antioxidant activity was investigated during seed germination. With the exception of beta-tocotrienol, all vitamin E isomers were detected in germinating seeds expressing OsHGGT. The antioxidant properties of germinating seed extracts were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals and lipid peroxidation (TBARS). Compared with intact wild-type seeds, transgenic seeds showed increases in radical scavenging of 5.4-17 and 23.2-35.3% in the DPPH and ABTS assays, respectively. Furthermore, the lipid peroxidation levels were 2.0-4.5-fold lower in germinating seeds from transgenic lines than in wild-type seeds. Therefore, it appears that the antioxidant potential of transgenic oil-producing plants such as soybean, sunflower, and corn may be enhanced by overexpressing OsHGGT during seed germination. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Antioxidant Activity and Inhibition of Lipid Peroxidation in Germinating Seeds of Transgenic Soybean Expressing OsHGGT | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/jf104012z | - |
dc.identifier.scopusid | 2-s2.0-78751563057 | - |
dc.identifier.wosid | 000286176100017 | - |
dc.identifier.bibliographicCitation | Journal of Agricultural and Food Chemistry, v.59, no.2, pp 584 - 591 | - |
dc.citation.title | Journal of Agricultural and Food Chemistry | - |
dc.citation.volume | 59 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 584 | - |
dc.citation.endPage | 591 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | VITAMIN-E | - |
dc.subject.keywordPlus | ALPHA-TOCOPHEROL | - |
dc.subject.keywordPlus | TOCOTRIENOLS | - |
dc.subject.keywordPlus | PLANTS | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | Tocotrienols | - |
dc.subject.keywordAuthor | tocopherols | - |
dc.subject.keywordAuthor | seed germination | - |
dc.subject.keywordAuthor | rice homogentisate geranylgeranyl transferase (OsHGGT) | - |
dc.subject.keywordAuthor | antioxidant activity | - |
dc.subject.keywordAuthor | lipid peroxidation | - |
dc.subject.keywordAuthor | soybean | - |
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