Effective Delivery of Antigen-Encapsulin Nanoparticle Fusions to Dendritic Cells Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection
DC Field | Value | Language |
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dc.contributor.author | Choi, Bongseo | - |
dc.contributor.author | Moon, Hyojin | - |
dc.contributor.author | Hong, Sung Joon | - |
dc.contributor.author | Shin, Changsik | - |
dc.contributor.author | Do, Yoonkyung | - |
dc.contributor.author | Ryu, Seongho | - |
dc.contributor.author | Kang, Sebyung | - |
dc.date.accessioned | 2021-09-10T05:24:20Z | - |
dc.date.available | 2021-09-10T05:24:20Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.issn | 1936-086X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/18645 | - |
dc.description.abstract | In cancer immunotherapy, robust and efficient activation of cytotoxic CD8(+) T cell immune responses is a promising, but challenging task. Dendritic cells (DCs) are well-known professional antigen presenting cells that initiate and regulate antigen-specific cytotoxic CD8(+) T cells that kill their target cells directly as well as secrete IFN-gamma, a cytokine critical in tumor rejection. Here, we employed recently established protein cage nano particles, encapsulin (Encap), as antigenic peptide nanocarriers by genetically incorporating the OT-1 peptide of ovalbumin (OVA) protein to the three different positions of the Encap subunit. With them, we evaluated their efficacy in activating DC-mediated antigen-specific T cell cytotoxicity and consequent melanoma tumor rejection in vivo. DCs efficiently engulfed Encap and its variants (OT-1-Encaps), which carry antigenic peptides at different positions, and properly processed them within phagosomes. Delivered OT-1 peptides were effectively presented by DCs to naive CD8(+) T cells successfully, resulting in the proliferation of antigen-specific cytotoxic CD8(+) T cells. OT-1-Encap vaccinations in B16-OVA melanoma tumor bearing mice effectively activated OT-1 peptide specific cytotoxic CD8(+) T cells before or even after tumor generation, resulting in significant suppression of tumor growth in prophylactic as well as therapeutic treatments. A large number of cytotoxic CD8(+) T cells that actively produce both intracellular and secretory IFN-gamma were observed in tumor-infiltrating lymphocytes collected from B16-OVA tumor masses originally vaccinated with OT-1-Encap-C upon tumor challenges. The approaches we describe herein may provide opportunities to develop epitope-dependent vaccination systems that stimulate and/or modulate efficient and epitope-specific cytotoxic T cell immune responses in nonpathogenic diseases. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Effective Delivery of Antigen-Encapsulin Nanoparticle Fusions to Dendritic Cells Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsnano.5b08084 | - |
dc.identifier.scopusid | 2-s2.0-84983441961 | - |
dc.identifier.wosid | 000381959100013 | - |
dc.identifier.bibliographicCitation | ACS Nano, v.10, no.8, pp 7339 - 7350 | - |
dc.citation.title | ACS Nano | - |
dc.citation.volume | 10 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 7339 | - |
dc.citation.endPage | 7350 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | VIRUS-LIKE PARTICLES | - |
dc.subject.keywordPlus | PROTEIN CAGE NANOPARTICLES | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MAGNETIC-RESONANCE | - |
dc.subject.keywordPlus | TARGETED DELIVERY | - |
dc.subject.keywordPlus | MOSAIC-VIRUS | - |
dc.subject.keywordPlus | MODULAR NANOPLATFORM | - |
dc.subject.keywordPlus | VIRAL NANOPARTICLES | - |
dc.subject.keywordPlus | PEPTIDE VACCINATION | - |
dc.subject.keywordAuthor | encapsulin | - |
dc.subject.keywordAuthor | antigen delivery | - |
dc.subject.keywordAuthor | vaccination | - |
dc.subject.keywordAuthor | dendritic cells | - |
dc.subject.keywordAuthor | cytotoxic T cells | - |
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