벤치규모 생물반응기에서 Shinella granuli CK-4에 의한 1,4-Dioxane의 생분해에 영향을 미치는 물리화학적 요인

Abstract

Our previous research has demonstrated that Shinella granuli CK-4 is capable of utilizing 1,4-dioxane as its sole source of carbon and energy when isolated from industrial wastewater [1]. In this study, we have extended this work to investigate the relationships between 1,4-dioxane degradation and the strain CK-4 and several relevant physicochemical environmental parameters in 3 L bench-scale bioreactors. One gram of 1,4-dioxane per liter was completely degraded within 96 hours of incubation, and the optimum temperature for 1,4-dioxane degradation was 30oC. 1,4-Dioxane degradation by CK-4 gradually increased as a function of airflow rate and agitation speed, and the optimum airflow rate and agitation speed were 2.5 L/min and 500 rotation per minutes, respectively, in this study. The addition of 50 mg/L tetrahydrofuran as a supplemental carbon source was found to stimulate the maximal bacterial growth and 1,4-dioxane degradation. The optimum results obtained from these experiments were combined and applied for 1,4-dioxane degradation. As a result, the complete degradation of 1,4-dioxane was achieved with the CK-4 culture within 72 hours of incubation. We explored the feasibility of using the cultures of S. granuli CK-4 for the degradation of 1,4-dioxane with the aim of microbial application in industrial wastewater treatment in bench-scale bioreactors.