Microenvironmental changes induced by placenta-derived mesenchymal stem cells restore ovarian function in ovariectomized rats via activation of the PI3K-FOXO3 pathway
DC Field | Value | Language |
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dc.contributor.author | Choi, Jong Ho | - |
dc.contributor.author | Seok, Jin | - |
dc.contributor.author | Lim, Seung Mook | - |
dc.contributor.author | Kim, Tae Hee | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.date.accessioned | 2021-09-10T06:46:49Z | - |
dc.date.available | 2021-09-10T06:46:49Z | - |
dc.date.issued | 2020-11-16 | - |
dc.identifier.issn | 1757-6512 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19392 | - |
dc.description.abstract | Background Translational studies have explored the therapeutic potential and feasibility of mesenchymal stem cells (MSCs) in several degenerative diseases; however, mechanistic studies of the function of these cells have been insufficient. As ovarian failure causes anovulation as well as ovarian steroid hormonal imbalances, the specific aims of this study were to analyze the therapeutic role of placenta-derived MSCs (PD-MSCs) in an ovarian failure ovariectomy (OVX) rat model and evaluate whether PD-MSC transplantation (Tx) improved folliculogenesis and oocyte maturation in the injured ovary through PI3K/Akt and FOXO signaling. Methods Blood and ovary tissue were collected and analyzed after various PD-MSC Tx treatments in an ovariectomized rat model. Changes in the expression of folliculogenesis- and ovary regeneration-related genes induced by PD-MSC treatments were analyzed by qRT-PCR, Western blotting, and histological analysis. Results The levels of hormones related to ovary function were significantly increased in the PD-MSC Tx groups compared with those in the nontransplantation group (NTx). The follicle numbers in the ovarian tissues were increased along with the increased expression of genes related to folliculogenesis in the PD-MSC Tx groups compared with the NTx groups. Furthermore, Tx PD-MSCs induced follicle maturation by increasing the phosphorylation of GSK3 beta and FOXO3 (p < 0.05) and shifting the balance of growth and apoptosis in oocytes. Conclusions Taken together, these results show that PD-MSC Tx can restore ovarian function and induce ovarian folliculogenesis via the PI3K/Akt and FOXO signaling pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central | - |
dc.title | Microenvironmental changes induced by placenta-derived mesenchymal stem cells restore ovarian function in ovariectomized rats via activation of the PI3K-FOXO3 pathway | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s13287-020-02002-0 | - |
dc.identifier.scopusid | 2-s2.0-85096058731 | - |
dc.identifier.wosid | 000594416800006 | - |
dc.identifier.bibliographicCitation | Stem Cell Research & Therapy, v.11, no.1 | - |
dc.citation.title | Stem Cell Research & Therapy | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | OOCYTES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | FERTILITY | - |
dc.subject.keywordPlus | FOLLICLES | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | FAILURE | - |
dc.subject.keywordAuthor | Placenta-derived stem cells | - |
dc.subject.keywordAuthor | Ovary function | - |
dc.subject.keywordAuthor | Oocyte survival | - |
dc.subject.keywordAuthor | PI3K | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | FOXO3a | - |
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