Effects of Vitamin D and Dexamethasone on Lymphocyte Proportions and Their Associations With Serum Concentrations of 25-Hydroxyvitamin D-3 In Vitro in Patients With Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Eun Bin | - |
dc.contributor.author | Shin, Jong Hwa | - |
dc.contributor.author | Kwon, Soonwook | - |
dc.contributor.author | Kim, Juhyeon | - |
dc.contributor.author | Seok, Jin Myoung | - |
dc.contributor.author | Kim, Byoung Joon | - |
dc.contributor.author | Min, Ju-Hong | - |
dc.date.accessioned | 2021-10-05T04:42:54Z | - |
dc.date.available | 2021-10-05T04:42:54Z | - |
dc.date.issued | 2021-07-29 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19869 | - |
dc.description.abstract | Background Clear associations have been found between vitamin D deficiency and several autoimmune diseases including multiple sclerosis (MS). However, the benefits of vitamin D supplementation on disease management remain a matter of debate. Objective and Methods Patients with MS (N=12) and neuromyelitis optica spectrum disorder (NMOSD; N=12) were enrolled along with 15 healthy controls. Changes in lymphocyte subset proportions during stimulation of their peripheral blood mononuclear cells (PBMCs) with the active form of vitamin D, 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3), and correlations with serum concentrations of the vitamin D precursor 25-hydroxyvitamin D-3 (serum 25(OH)D-3) were explored. The impact of 1,25(OH)(2)D-3 stimulation on the expression of vitamin-D-responsive genes in immune cells was also investigated. Results In both MS and NMOSD, stimulation of PBMCs with 1,25(OH)(2)D-3 followed by steroid suppressed the proliferation of total lymphocytes and T cells. The ratio of CD19(+)CD27(+) memory B cells (Bmem) to all B cells after stimulation with 1,25(OH)(2)D-3 was negatively correlated with serum 25(OH)D-3 in MS (Spearman's rho=-0.594, p=0.042), but positively correlated in NMOSD (Pearson's r = 0.739, p=0.006). However, there was no relationship between the ratio of Bmem to CD19(+)CD24(+)CD38(+) regulatory B cells and serum 25(OH)D-3 in either MS or NMOSD. In addition, the level of 1,25(OH)(2)D-3-induced CYP24A1 mRNA expression in PBMCs was significantly and negatively correlated with serum 25(OH)D-3 (for Delta C-T, r=0.744, p=0.014) in MS. Conclusion These findings suggest a beneficial impact of stimulation of PBMCs with vitamin D followed by steroid on the T-cell population. The association between patient serum 25(OH)D-3 and the proportion of Bmem under immune-cell stimulation differed between MS and NMOSD. Further investigations are warranted with larger patient populations. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Frontiers Media S.A. | - |
dc.title | Effects of Vitamin D and Dexamethasone on Lymphocyte Proportions and Their Associations With Serum Concentrations of 25-Hydroxyvitamin D-3 In Vitro in Patients With Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3389/fimmu.2021.677041 | - |
dc.identifier.scopusid | 2-s2.0-85112424352 | - |
dc.identifier.wosid | 000684968200001 | - |
dc.identifier.bibliographicCitation | Frontiers in Immunology, v.12 | - |
dc.citation.title | Frontiers in Immunology | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | B-CELLS | - |
dc.subject.keywordPlus | 1,25-DIHYDROXYVITAMIN D-3 | - |
dc.subject.keywordPlus | MEMORY | - |
dc.subject.keywordPlus | BALANCE | - |
dc.subject.keywordAuthor | vitamin D | - |
dc.subject.keywordAuthor | lymphocytes | - |
dc.subject.keywordAuthor | memory B cells | - |
dc.subject.keywordAuthor | multiple sclerosis | - |
dc.subject.keywordAuthor | neuromyelitis optica spectrum disorder | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.