Heparin-Mimicking Polymer-Based In Vitro Platform Recapitulates In Vivo Muscle Atrophy Phenotypes
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunbum | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Fendereski, Mona | - |
dc.contributor.author | Lee, Hyo-Shin | - |
dc.contributor.author | Kang, Da Yeon | - |
dc.contributor.author | Hur, Sung Sik | - |
dc.contributor.author | Amirian, Jhaleh | - |
dc.contributor.author | Kim, Yunhye | - |
dc.contributor.author | Pham, Nghia Thi | - |
dc.contributor.author | Suh, Nayoung | - |
dc.contributor.author | Hwang, Nathaniel Suk-Yeon | - |
dc.contributor.author | Ryu, Seongho | - |
dc.contributor.author | Yoon, Jeong Kyo | - |
dc.contributor.author | Hwang, Yongsung | - |
dc.date.accessioned | 2021-10-05T04:43:32Z | - |
dc.date.available | 2021-10-05T04:43:32Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19894 | - |
dc.description.abstract | The cell-cell/cell-matrix interactions between myoblasts and their extracellular microenvironment have been shown to play a crucial role in the regulation of in vitro myogenic differentiation and in vivo skeletal muscle regeneration. In this study, by harnessing the heparin-mimicking polymer, poly(sodium-4-styrenesulfonate) (PSS), which has a negatively charged surface, we engineered an in vitro cell culture platform for the purpose of recapitulating in vivo muscle atrophy-like phenotypes. Our initial findings showed that heparin-mimicking moieties inhibited the fusion of mononucleated myoblasts into multinucleated myotubes, as indicated by the decreased gene and protein expression levels of myogenic factors, myotube fusion-related markers, and focal adhesion kinase (FAK). We further elucidated the underlying molecular mechanism via transcriptome analyses, observing that the insulin/PI3K/mTOR and Wnt signaling pathways were significantly downregulated by heparin-mimicking moieties through the inhibition of FAK/Cav3. Taken together, the easy-to-adapt heparin-mimicking polymer-based in vitro cell culture platform could be an attractive platform for potential applications in drug screening, providing clear readouts of changes in insulin/PI3K/mTOR and Wnt signaling pathways. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Heparin-Mimicking Polymer-Based In Vitro Platform Recapitulates In Vivo Muscle Atrophy Phenotypes | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/ijms22052488 | - |
dc.identifier.scopusid | 2-s2.0-85101688792 | - |
dc.identifier.wosid | 000628283400001 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.22, no.5 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 22 | - |
dc.citation.number | 5 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordAuthor | synthetic mimic of heparin | - |
dc.subject.keywordAuthor | poly(sodium-4-styrenesulfonate) | - |
dc.subject.keywordAuthor | myoblast | - |
dc.subject.keywordAuthor | myogenic differentiation | - |
dc.subject.keywordAuthor | fusion | - |
dc.subject.keywordAuthor | focal adhesion kinase (FAK) | - |
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