Molecular cloning, sequence characterization, and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn, Macrobrachium rosenbergii
DC Field | Value | Language |
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dc.contributor.author | Baliarsingh, Snigdha | - |
dc.contributor.author | Sahoo, Sonalina | - |
dc.contributor.author | Jo, Yong Hun | - |
dc.contributor.author | Han, Yeon Soo | - |
dc.contributor.author | Sarkar, Arup | - |
dc.contributor.author | Lee, Yong Seok | - |
dc.contributor.author | Mohanty, Jyotirmaya | - |
dc.contributor.author | Patnaik, Bharat Bhusan | - |
dc.date.accessioned | 2022-04-21T02:40:21Z | - |
dc.date.available | 2022-04-21T02:40:21Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 0967-6120 | - |
dc.identifier.issn | 1573-143X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/20658 | - |
dc.description.abstract | Lectin protein families are diverse and multi-functional in crustaceans. The carbohydrate-binding domains (CRDs) of lectins recognize the molecular patterns associated with pathogens and orchestrate important roles in crustacean defense. In this study, two lectin homologs, a single CRD containing C-type lectin (CTL) and an L-type lectin (LTL) domain containing endoplasmic reticulum Golgi intermediate compartment 53 kDa protein (ERGIC-53) were identified from the freshwater prawn, Macrobrachium rosenbergii. The open reading frames of MrCTL and MrERGIC-53 were 654 and 1,515 bp, encoding polypeptides of 217 and 504 amino acids, respectively. Further, MrCTL showed a 20-amino acid transmembrane helix region and 10 carbohydrate-binding residues within the CRD. MrERGIC-53 showed a signal peptide region, a type-I transmembrane region, and a coiledcoil region at the C-terminus. Phylogenetic analysis revealed a close relationship between MrCTL and MrLectin and M. nipponense CTL (MnCTL), whereas MrERGIC-53 shared high sequence identity with Eriocheir sinensis ERGIC-53 and Penaeus vannamei MBL-1. A homology-based model predicted small carbohydrate-combining sites with a metal-binding site for ligand binding (Ca2+ binding site) in MrCTL and beta-sheets connected by short loops and beta-bends forming a dome-shaped beta-barrel structure representing the LTL domain of MrERGIC-53. Quantitative real-time polymerase chain reaction detected MrCTL and MrERGIC-53 transcripts in all examined tissues, with particularly high levels observed in hemocytes, hepatopancreas, and mucosal-associated tissues, such as the stomach and intestine. Further, the expression levels of MrCTL and MrERGIC-53 transcripts were remarkably altered after V. harveyi challenge, suggesting putative function in host innate immunity. | - |
dc.format.extent | 25 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Kluwer Academic Publishers | - |
dc.title | Molecular cloning, sequence characterization, and expression analysis of C-type lectin (CTL) and ER-Golgi intermediate compartment 53-kDa protein (ERGIC-53) homologs from the freshwater prawn, Macrobrachium rosenbergii | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1007/s10499-022-00845-3 | - |
dc.identifier.scopusid | 2-s2.0-85124273590 | - |
dc.identifier.wosid | 000751200200001 | - |
dc.identifier.bibliographicCitation | Aquaculture International, v.30, no.2, pp 1011 - 1035 | - |
dc.citation.title | Aquaculture International | - |
dc.citation.volume | 30 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1011 | - |
dc.citation.endPage | 1035 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.subject.keywordPlus | SHRIMP LITOPENAEUS-VANNAMEI | - |
dc.subject.keywordPlus | INNATE IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | MIGHT | - |
dc.subject.keywordAuthor | Macrobrachium rosenbergii | - |
dc.subject.keywordAuthor | CTL | - |
dc.subject.keywordAuthor | ERGIC-53 | - |
dc.subject.keywordAuthor | Immune response | - |
dc.subject.keywordAuthor | V. harveyi | - |
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