Improved Productivity of Astaxanthin from Photosensitive Haematococcus pluvialis Using Phototaxis Technology
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kang Hyun | - |
dc.contributor.author | Chun, Youngsang | - |
dc.contributor.author | Lee, Ja Hyun | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Yoo, Hah Young | - |
dc.contributor.author | Kwak, Ho Seok | - |
dc.date.accessioned | 2022-05-03T02:40:20Z | - |
dc.date.available | 2022-05-03T02:40:20Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1660-3397 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/20713 | - |
dc.description.abstract | Haematococcus pluvialis is a microalgae actively studied for the production of natural astaxanthin, which is a powerful antioxidant for human application. However, it is economically disadvantageous for commercialization owing to the low productivity of astaxanthin. This study reports an effective screening strategy using the negative phototaxis of the H. pluvialis to attain the mutants having high astaxanthin production. A polydimethylsiloxane (PDMS)-based microfluidic device irradiated with a specific light was developed to efficiently figure out the phototactic response of H. pluvialis. The partial photosynthesis deficient (PP) mutant (negative control) showed a 0.78-fold decreased cellular response to blue light compared to the wild type, demonstrating the positive relationship between the photosynthetic efficiency and the phototaxis. Based on this relationship, the Haematococcus mutants showing photosensitivity to blue light were selected from the 10,000 random mutant libraries. The M1 strain attained from the phototaxis-based screening showed 1.17-fold improved growth rate and 1.26-fold increases in astaxanthin production (55.12 +/- 4.12 mg g(-1)) in the 100 L photo-bioreactor compared to the wild type. This study provides an effective selection tool for industrial application of the H. pluvialis with improved astaxanthin productivity. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Improved Productivity of Astaxanthin from Photosensitive Haematococcus pluvialis Using Phototaxis Technology | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/md20040220 | - |
dc.identifier.scopusid | 2-s2.0-85127764272 | - |
dc.identifier.wosid | 000785165300001 | - |
dc.identifier.bibliographicCitation | Marine Drugs, v.20, no.4, pp 1 - 12 | - |
dc.citation.title | Marine Drugs | - |
dc.citation.volume | 20 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | HYPERPRODUCING MUTANTS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | ALGA | - |
dc.subject.keywordPlus | CULTIVATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | CHLOROPHYCEAE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordAuthor | Haematococcus pluvialis | - |
dc.subject.keywordAuthor | astaxanthin | - |
dc.subject.keywordAuthor | photosynthetic efficiency | - |
dc.subject.keywordAuthor | microfluidics | - |
dc.subject.keywordAuthor | photobioreactor | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.