Biochanin A induces a brown-fat phenotype via improvement of mitochondrial biogenesis and activation ofAMPKsignaling in murineC3H10T1/2 mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.author | Rahman, Md. Shamim | - |
dc.contributor.author | Imran, Khan Mohammad | - |
dc.contributor.author | Hossain, Monir | - |
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Kim, Yong-Sik | - |
dc.date.accessioned | 2021-08-11T08:30:10Z | - |
dc.date.available | 2021-08-11T08:30:10Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.issn | 0951-418X | - |
dc.identifier.issn | 1099-1573 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2073 | - |
dc.description.abstract | In this study, we investigated the effect of Biochanin A (BioA), an O-methylated isoflavone on the brown-fat phenotype formation and on the associated thermogenic program including mitochondrial biogenesis and lipolysis in C3H10T1/2 MSCs. Our data demonstrates that Treatment with BioA in an adipogenic differentiation cocktail induced formation of brown-fat-like adipocytes from C3H10T1/2 MSCs without treatment with a known browning inducer (rosiglitazone or T3) at an early stage of differentiation. The formation of brown-fat-like adipocytes by BioA treatment was evidenced by upregulation of key thermogenic markers:Ucp1,Pgc1 alpha,Prdm16, andPpar gamma. BioA also increased the expression of beige (Cd137andFgf21) and brown (Elovl3andZic1)-specific markers. Additionally, BioA treatment promoted mitochondrial biogenesis, judging by the upregulation of genes;Cox8b,Cidea,Dio2,Sirt1,Opa1, andFis1. BioA treatment increased the amount of mitochondrial DNA and its encoded proteins: oxidative phosphorylation complexes (I-V); this change was associated with high oxygen consumption by C3H10T1/2 MSCs. A small-interfering-RNA-induced gene knockdown and experiments with dorsomorphin-driven competitive inhibition revealed that BioA exerts the thermogenic action via activation of AMPK signaling. Our study shows the mechanism of BioA-induced promotion of a brown-fat phenotype. Nonetheless, clinical research is necessary to validate BioA as a brown-fat-like signature inducer. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Biochanin A induces a brown-fat phenotype via improvement of mitochondrial biogenesis and activation ofAMPKsignaling in murineC3H10T1/2 mesenchymal stem cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/ptr.6845 | - |
dc.identifier.scopusid | 2-s2.0-85089786409 | - |
dc.identifier.wosid | 000562217000001 | - |
dc.identifier.bibliographicCitation | Phytotherapy Research, v.35, no.2, pp 920 - 931 | - |
dc.citation.title | Phytotherapy Research | - |
dc.citation.volume | 35 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 920 | - |
dc.citation.endPage | 931 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | WHITE ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | AMPK | - |
dc.subject.keywordPlus | LIPOLYSIS | - |
dc.subject.keywordPlus | ADIPOCYTES | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ISOFLAVONE | - |
dc.subject.keywordPlus | DESNUTRIN/ATGL | - |
dc.subject.keywordPlus | AGONISTS | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | biochanin A | - |
dc.subject.keywordAuthor | brown-fat phenotype | - |
dc.subject.keywordAuthor | 2 | - |
dc.subject.keywordAuthor | lipolysis | - |
dc.subject.keywordAuthor | mitochondrial biogenesis | - |
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