Protective effect of GK2 fused BLVRA protein against oxidative stress-induced dopaminergic neuronal cell damage
DC Field | Value | Language |
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dc.contributor.author | Choi, Yeon Joo | - |
dc.contributor.author | Kwon, Hyun Jung | - |
dc.contributor.author | Shin, Min Jea | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Youn, Gi Soo | - |
dc.contributor.author | Park, Jung Hwan | - |
dc.contributor.author | Yeo, Hyeon Ji | - |
dc.contributor.author | Yeo, Eun Ji | - |
dc.contributor.author | Kim, Hyeong Seop | - |
dc.contributor.author | Lee, Lee Re | - |
dc.contributor.author | Kim, Na Yeon | - |
dc.contributor.author | Kwon, Su Yeon | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Kim, Gun Woo | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Han, Kyu Hyung | - |
dc.contributor.author | Lee, Keun Wook | - |
dc.contributor.author | Park, Jong Kook | - |
dc.contributor.author | Lee, Chan Hee | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2023-03-09T07:41:02Z | - |
dc.date.available | 2023-03-09T07:41:02Z | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 1742-464X | - |
dc.identifier.issn | 1742-4658 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/22169 | - |
dc.description.abstract | It is well known that oxidative stress is highly associated with Parkinson's disease (PD), and biliverdin reductase A (BLVRA) is known to have antioxidant properties against oxidative stress. In this study, we developed a novel N-acetylgalactosamine kinase (GK2) protein transduction domain (PTD) derived from adenosine A2A and fused with BLVRA to determine whether the GK2-BLVRA fusion protein could protect dopaminergic neuronal cells (SH-SY5Y) from oxidative stress in vitro and in vivo using a PD animal model. GK2-BLVRA was transduced into various cells, including SH-SY5Y cells, without cytotoxic effects, and this fusion protein protected SH-SY5Y cells and reduced reactive oxygen species production and DNA damage after 1-methyl-4-phenylpyridinium (MPP+) exposure. GK2-BLVRA suppressed mitogen-activated protein kinase (MAPK) activation and modulated apoptosis-related protein (Bcl-2, Bax, cleaved Caspase-3 and -9) expression levels. In the PD animal model, GK2-BLVRA transduced into the substantia nigra crossed the blood-brain barrier and markedly reduced dopaminergic neuronal cell death in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced animals. These results indicate that our novel PTD GK-2 is useful for the transduction of protein, and GK2-BLVRA exhibits a beneficial effect against dopaminergic neuronal cell death in vitro and in vivo, suggesting that BLVRA can be used as a therapeutic agent for PD. | - |
dc.format.extent | 16 | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.title | Protective effect of GK2 fused BLVRA protein against oxidative stress-induced dopaminergic neuronal cell damage | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/febs.16721 | - |
dc.identifier.scopusid | 2-s2.0-85147492903 | - |
dc.identifier.wosid | 000928990600001 | - |
dc.identifier.bibliographicCitation | FEBS Journal, v.290, no.11, pp 2923 - 2938 | - |
dc.citation.title | FEBS Journal | - |
dc.citation.volume | 290 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2923 | - |
dc.citation.endPage | 2938 | - |
dc.type.docType | Article; Early Access | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | HUMAN BILIVERDIN REDUCTASE | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSE | - |
dc.subject.keywordPlus | ALZHEIMER-DISEASE | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | MAPK | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordAuthor | GK2-BLVRA | - |
dc.subject.keywordAuthor | MAPK | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | Parkinson's disease | - |
dc.subject.keywordAuthor | protein therapy | - |
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