Genetic diversity and population structure of the endangered fishPseudobagrus brevicorpus(Bagridae) using a newly developed 12-microsatellite marker

Abstract

Background Pseudobagrus brevicorpusis an endangered species in Korea. The development of genetic markers and genetic information regarding the populations of this species are needed to establish conservation strategies. Objective As part of the conservation ofP. brevicorpus, a 12-microsatellite marker was developed using next-generation sequencing (NGS) to provide current genetic population information. Methods Microsatellites fromP. brevicorpuswere identified by NGS analysis. Genetic diversity and genetic structure analysis of six populations (Seojeong Stream [SJ], Gokgang Stream [GK], Jaho Stream [JH], Daega Stream [DG], Nam River [NG], and Deokcheon River [DC]) ofP. brevicorpuswere conducted using the newly developed microsatellite marker. Results NGS generated 10,347,578 reads and identified 659,507 simple sequence repeats. Twelve microsatellites were successfully amplified and verified in 30 individuals ofP. brevicorpus. The genetic diversity of the sixP. brevicorpuspopulations in terms of the number of alleles ranged from 3.667 to 7.111. All populations except DG deviated significantly from Hardy-Weinberg equilibrium (HWE) at one or more loci. The genetic distances of the six populations showed the closest relationship between the SJ and GK populations (independent Stream populations), and there was a close relationship with the JH population among the Nakdong River. Structure analysis showed thatP. brevicorpusis largely divided into two groups. Conclusions The developed microsatellite marker will be used to provide basic genetic data ofP. brevicorpus. Genetic diversity and structure analysis of the population will provide useful information for conservation management ofP. brevicorpus.