Helicobacter pylori infection induces STAT3 phosphorylation on Ser727 and autophagy in human gastric epithelial cells and mouse stomach
DC Field | Value | Language |
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dc.contributor.author | Piao, Juan-Yu | - |
dc.contributor.author | Kim, Su-Jung | - |
dc.contributor.author | Kim, Do-Hee | - |
dc.contributor.author | Park, Ji Hyun | - |
dc.contributor.author | Park, Sin-Aye | - |
dc.contributor.author | Han, Hyeong-jun | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Yoon, Kichul | - |
dc.contributor.author | Lee, Ha-Na | - |
dc.contributor.author | Kim, Nayoung | - |
dc.contributor.author | Hahm, Ki Baik | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2021-08-11T08:32:47Z | - |
dc.date.available | 2021-08-11T08:32:47Z | - |
dc.date.issued | 2020-09-24 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2464 | - |
dc.description.abstract | Helicobacter pylori (H. pylori) infection is considered as one of the principal risk factors of gastric cancer. Constitutive activation of the signal transducer and activator of transcription 3 (STAT3) plays an important role in inflammation-associated gastric carcinogenesis. In the canonical STAT3 pathway, phosphorylation of STAT3 on Tyr705 is a major event of STAT3 activation. However, recent studies have demonstrated that STAT3 phosphorylated on Ser727 has an independent function in mitochondria. In the present study, we found that human gastric epithelial AGS cells infected with H. pylori resulted in localization of STAT3 phosphorylated on Ser727 (P-STAT3(Ser727)), predominantly in the mitochondria. Notably, H. pylori-infected AGS cells exhibited the loss of mitochondrial integrity and increased expression of the microtubule-associated protein light chain 3 (LC3), the autophagosomal membrane-associated protein. Treatment of AGS cells with a mitophagy inducer, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), resulted in accumulation of P-STAT3(Ser727) in mitochondria. In addition, the elevated expression and mitochondrial localization of LC3 induced by H. pylori infection were attenuated in AGS cells harboring STAT3 mutation defective in Ser727 phosphorylation (S727A). We also observed that both P-STAT3(Ser727) expression and LC3 accumulation were increased in the mitochondria of H. pylori-inoculated mouse stomach. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Helicobacter pylori infection induces STAT3 phosphorylation on Ser727 and autophagy in human gastric epithelial cells and mouse stomach | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/s41598-020-72594-3 | - |
dc.identifier.scopusid | 2-s2.0-85091414759 | - |
dc.identifier.wosid | 000608592000004 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.10, no.1 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | MITOCHONDRIAL STAT3 | - |
dc.subject.keywordPlus | SIGNAL TRANSDUCER | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | GRIM-19 | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | ACTIVATOR | - |
dc.subject.keywordPlus | TYROSINE | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordAuthor | Helicobacter pylori | - |
dc.subject.keywordAuthor | STAT3 | - |
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