Chlorin e6-mediated photodynamic therapy modulates adipocyte differentiation and lipogenesis in 3T3-L1 cells
DC Field | Value | Language |
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dc.contributor.author | Ryu, A-Reum | - |
dc.contributor.author | Kim, Yong-Wan | - |
dc.contributor.author | Lee, Mi-Young | - |
dc.date.accessioned | 2021-08-11T08:32:55Z | - |
dc.date.available | 2021-08-11T08:32:55Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1572-1000 | - |
dc.identifier.issn | 1873-1597 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2503 | - |
dc.description.abstract | Background: Chlorin e6-mediated photodynamic therapy (Ce6-mediated PDT) is a therapeutic modality that consists of a photosensitizer, source of light at a suitable wavelength, and molecular oxygen. PDT has been widely used for the treatment of cancers and non-neoplastic disorders. However, information on the inhibitory activity of Ce6-mediated PDT on adipogenesis and lipogenesis in 3T3-L1 cells is not available to date. Methods: MTT assay, Oil Red O staining, and Nile red staining were used to investigate lipid accumulation in 3T3-L1 cells to clarify adipocyte differentiation. The expression levels of various molecular signals associated with adipogenesis and lipogenesis were examined by RT-PCR and western blot analysis. Results: Adipocyte differentiation and lipid accumulation were reduced by Ce6-mediated PDT in 3T3-L1 cells. Inhibition of the expression of PPAR-gamma, C/EBP alpha, beta, delta, SREBP-1, FAS, and LPL and the activation of AMPK, revealed concise modulation of adipogenic and lipogenic molecules by Ce6-mediated PDT. The anti-adipogenic effect of Ce6-mediated PDT was reversed by AMPK alpha siRNA. Conclusion: Ce6-mediated PDT inhibits adipocyte differentiation and lipogenesis via regulating AMPK. Ce6-mediated PDT might serve as a potential therapy for the treatment of obesity and obesity-associated diseases. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Chlorin e6-mediated photodynamic therapy modulates adipocyte differentiation and lipogenesis in 3T3-L1 cells | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.pdpdt.2020.101917 | - |
dc.identifier.scopusid | 2-s2.0-85088516233 | - |
dc.identifier.wosid | 000576942700005 | - |
dc.identifier.bibliographicCitation | Photodiagnosis and Photodynamic Therapy, v.31 | - |
dc.citation.title | Photodiagnosis and Photodynamic Therapy | - |
dc.citation.volume | 31 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | HALOGEN LIGHT | - |
dc.subject.keywordPlus | PREADIPOCYTES | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | AMPK | - |
dc.subject.keywordPlus | E6 | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | SUPPRESSES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Chlorin e6 | - |
dc.subject.keywordAuthor | LED | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Adipocyte | - |
dc.subject.keywordAuthor | Lipogenesis | - |
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