Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategy
DC Field | Value | Language |
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dc.contributor.author | Jiang, Kunlun | - |
dc.contributor.author | Lee, Jung-Hoon | - |
dc.contributor.author | Fung, To Sing | - |
dc.contributor.author | Wu, Jingrui | - |
dc.contributor.author | Liu, Congnuan | - |
dc.contributor.author | Mi, Hua | - |
dc.contributor.author | Rajapakse, R. P. V. Jayanthe | - |
dc.contributor.author | Balasuriya, Udeni B. R. | - |
dc.contributor.author | Peng, Yung-Kang | - |
dc.contributor.author | Go, Yun Young | - |
dc.date.accessioned | 2023-12-14T06:31:11Z | - |
dc.date.available | 2023-12-14T06:31:11Z | - |
dc.date.issued | 2023-09 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.issn | 1873-4324 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25467 | - |
dc.description.abstract | The current global COVID-19 pandemic once again highlighted the urgent need for a simple, cost-effective, and sensitive diagnostic platform that can be rapidly developed for distribution and easy access in resource-limited areas. Here, we present a simple and low-cost plasmonic photothermal (PPT)-reverse transcription-colorimetric polymerase chain reaction (RTcPCR) for molecular diagnosis of dengue virus (DENV) infection. The assay can be completed within 54 min with an estimated detection limit of 1.6 copies/mu L of viral nucleic acid. The analytical sensitivity and specificity of PPT-RTcPCR were comparable to that of the reference RT-qPCR assay. Moreover, the clinical performance of PPT-RTcPCR was evaluated and validated using 158 plasma samples collected from patients suspected of dengue infection. The results showed a diagnostic agreement of 97.5% compared to the reference RT-qPCR and demonstrated a clinical sensitivity and specificity of 97.0% and 100%, respectively. The simplicity and reliability of our PPT-RTcPCR strategy suggest it can provide a foundation for developing a field -deployable diagnostic assay for dengue and other infectious diseases. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER | - |
dc.title | Next-generation diagnostic test for dengue virus detection using an ultrafast plasmonic colorimetric RT-PCR strategy | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.aca.2023.341565 | - |
dc.identifier.scopusid | 2-s2.0-85163831976 | - |
dc.identifier.wosid | 001030643600001 | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, v.1274 | - |
dc.citation.title | ANALYTICA CHIMICA ACTA | - |
dc.citation.volume | 1274 | - |
dc.type.docType | Article; Early Access | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | INFECTIOUS-DISEASES | - |
dc.subject.keywordPlus | POINT | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Plasmonic photothermal PCR | - |
dc.subject.keywordAuthor | Photothermal effect | - |
dc.subject.keywordAuthor | Colorimetric | - |
dc.subject.keywordAuthor | Dengue diagnosis | - |
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