Electrochemical DNA Cleavage Sensing for EcoRV Activity and Inhibition with an ERGO Electrode
DC Field | Value | Language |
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dc.contributor.author | Oh, Da Eun | - |
dc.contributor.author | Kim, Hyun Beom | - |
dc.contributor.author | Kim, Tae Hyun | - |
dc.date.accessioned | 2024-06-11T07:02:22Z | - |
dc.date.available | 2024-06-11T07:02:22Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25926 | - |
dc.description.abstract | An electrochemically reduced graphene oxide (ERGO) electrode-based electrochemical assay was developed for rapid, sensitive, and straightforward analysis of both activity and inhibition of the endonuclease EcoRV. The procedure uses a DNA substrate designed for EcoRV, featuring a double-stranded DNA (dsDNA) region labeled with methylene blue (MB) and a single-stranded DNA (ssDNA) region immobilized on the ERGO surface. The ERGO electrode, immobilized with the DNA substrate, was subsequently exposed to a sample containing EcoRV. Upon enzymatic hydrolysis, the cleaved dsDNA fragments were detached from the ERGO surface, leading to a decrease in the MB concentration near the electrode. This diminished the electron transfer efficiency for MB reduction, resulting in a decreased reduction current. This assay demonstrates excellent specificity and high sensitivity, with a limit of detection (LOD) of 9.5 x 10-3 U mL-1. Importantly, it can also measure EcoRV activity in the presence of aurintricarboxylic acid, a known inhibitor, highlighting its potential for drug discovery and clinical diagnostic applications. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Electrochemical DNA Cleavage Sensing for EcoRV Activity and Inhibition with an ERGO Electrode | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/bios14020073 | - |
dc.identifier.scopusid | 2-s2.0-85185861104 | - |
dc.identifier.wosid | 001169877100001 | - |
dc.identifier.bibliographicCitation | BIOSENSORS-BASEL, v.14, no.2 | - |
dc.citation.title | BIOSENSORS-BASEL | - |
dc.citation.volume | 14 | - |
dc.citation.number | 2 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | SITE-DIRECTED MUTAGENESIS | - |
dc.subject.keywordPlus | REDUCED GRAPHENE OXIDE | - |
dc.subject.keywordPlus | RESTRICTION-ENDONUCLEASE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | RECOGNITION SITE | - |
dc.subject.keywordPlus | METHYLENE-BLUE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordAuthor | endonuclease | - |
dc.subject.keywordAuthor | EcoRV | - |
dc.subject.keywordAuthor | electrochemical assay | - |
dc.subject.keywordAuthor | graphene | - |
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