Development and validation of an LC-MS/MS method for determination of tetrodotoxin and its analogues in various pufferfish tissues and its application in Korean pufferfish
- Authors
- Park, Bong Ki; Lee, Hyunjun; Kim, Inhwan; Choi, Kwang-Sik; Lee, Wan-Ok; Kajino, Nobuhisa; Hong, Hyun-Ki; Lee, Hee-Seok; Choi, Changsun; Lee, Jihyun
- Issue Date
- Jan-2024
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Pufferfish; Takifugu pufferfish; Tetrodotoxin; Tetrodotoxin analogue; LC-MS/MS; Method validation
- Citation
- JOURNAL OF FOOD COMPOSITION AND ANALYSIS, v.125
- Journal Title
- JOURNAL OF FOOD COMPOSITION AND ANALYSIS
- Volume
- 125
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26072
- DOI
- 10.1016/j.jfca.2023.105816
- ISSN
- 0889-1575
1096-0481
- Abstract
- A robust method was developed and validated for analyzing tetrodotoxin and its analogues (TTXs) in high- and low-fat tissues of pufferfish using LC-MS/MS. The tissue distribution of TTXs in four Takifugu pufferfish species and their phenotypic variants was determined using the validated method. A significant matrix effect was shown, and the matrix-matched TTX calibration curves (r(2) > 0.999) were used for the analysis of TTX. The method detection limit for TTX ranged from 0.006 to 0.009 mu g/g. Recoveries from TTX-fortified tissue samples (0.1-2.0 mu g/g) were in the range of 96.1-107.3 % with RSDs less than 7.8 %. TTX levels in the pufferfish tissues reached a maximum of 1.32 x 10(4) mu g/g. The developed method was used for the relative quantification of TTX analogues. The major TTX analogues were deoxyTTXs (i.e., trideoxy-, dideoxy-, and deoxyTTX). The ovary and liver of pufferfish showed higher concentrations of TTXs compared to other tissues. The developed method was successfully validated and applied to Korean pufferfish samples.
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