Effects of sub-inhibitory concentrations of nafcillin, vancomycibipn, ciprofloxacin, and rifampin on biofilm formation of clinical methicillin-resistant <i>Staphylococcus aureus</i>
DC Field | Value | Language |
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dc.contributor.author | Park, Ki-Ho | - |
dc.contributor.author | Kim, Dokyoung | - |
dc.contributor.author | Jung, Minji | - |
dc.contributor.author | Kim, Dong Youn | - |
dc.contributor.author | Lee, Yu-Mi | - |
dc.contributor.author | Lee, Mi Suk | - |
dc.contributor.author | Hong, Kyung-Wook | - |
dc.contributor.author | Bae, In-Gyu | - |
dc.contributor.author | Hong, Sun In | - |
dc.contributor.author | Cho, Oh-Hyun | - |
dc.date.accessioned | 2024-06-11T08:30:48Z | - |
dc.date.available | 2024-06-11T08:30:48Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.issn | 2165-0497 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26267 | - |
dc.description.abstract | Methicillin-resistant Staphylococcus aureus (MRSA) infections are often difficult to treat because of their biofilm-forming ability and antimicrobial resistance. We investigated the effects of sub-minimal inhibitory concentrations (MICs) of antibiotics on MRSA biofilm formation. Clinical MRSA isolates were grown with sub-MICs (1/256-1/2 x MICs) of nafcillin, vancomycin, ciprofloxacin, and rifampin. The biofilm biomass was measured using crystal violet staining. Of the 107 MRSA isolates tested, 63 (58.9%) belonged to sequence type 5 (ST5), and 44 (41.1%) belonged to ST72. The MIC50/MIC90 values of nafcillin, vancomycin, ciprofloxacin, and rifampin were 256/512, 1/2, 64/512, and 0.008/0.03 mg/L, respectively. The sub-MICs of nafcillin, vancomycin, ciprofloxacin, and rifampin promoted biofilm formation in 75 (70.1%), 49 (45.8%), 89 (83.2%), and 89 (83.2%) isolates, respectively. At sub-MICs of nafcillin, the factors associated with strong biofilm induction were the ST5 strain (P = 0.001) and agr dysfunction (P = 0.005). For the sub-MICs of ciprofloxacin, the associated factors were the ST5 strain (P = 0.002), staphylococcal protein A type t002 strain (P < 0.001), and ciprofloxacin resistance (P < 0.001). Among the sub-MICs of rifampin, only ST5 was associated with strong biofilm induction (P = 0.006). Because the sub-MICs of rifampin were much lower than clinically relevant concentrations, we further tested the capability of biofilm induction in 0.03 - 32 mg/L of rifampin. At these concentrations, rifampin-induced biofilm formation was rare in rifampin-susceptible MRSA [1.0% (1 of 100)] but common in rifampin-resistant MRSA [71.4% (5 of 7), P < 0.001]. Induction of biofilm biomass at sub-MICs of antibiotics is common in clinical MRSA isolates and is differentially affected by the MRSA strain and antibiotic class. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Effects of sub-inhibitory concentrations of nafcillin, vancomycibipn, ciprofloxacin, and rifampin on biofilm formation of clinical methicillin-resistant <i>Staphylococcus aureus</i> | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1128/spectrum.03412-23 | - |
dc.identifier.wosid | 001206555000006 | - |
dc.identifier.bibliographicCitation | MICROBIOLOGY SPECTRUM | - |
dc.citation.title | MICROBIOLOGY SPECTRUM | - |
dc.type.docType | Article; Early Access | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SUBINHIBITORY CONCENTRATIONS | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | EPIDERMIDIS | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CLONES | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
dc.subject.keywordAuthor | biofilm | - |
dc.subject.keywordAuthor | nafcilllin | - |
dc.subject.keywordAuthor | vancomycin | - |
dc.subject.keywordAuthor | ciprofloxacin | - |
dc.subject.keywordAuthor | rifampin | - |
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