Type-Specific Viral Load and Physical State of HPV Type 16, 18, and 58 as Diagnostic Biomarkers for High-Grade Squamous Intraepithelial Lesions or Cervical Cancer
DC Field | Value | Language |
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dc.contributor.author | Kim, Jongseung | - |
dc.contributor.author | Kim, Bu Kyung | - |
dc.contributor.author | Jeon, Dongsoo | - |
dc.contributor.author | Lee, Chae Hyeong | - |
dc.contributor.author | Roh, Ju-Won | - |
dc.contributor.author | Kim, Joo-Young | - |
dc.contributor.author | Park, Sang-Yoon | - |
dc.date.accessioned | 2021-08-11T08:36:37Z | - |
dc.date.available | 2021-08-11T08:36:37Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 1598-2998 | - |
dc.identifier.issn | 2005-9256 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2968 | - |
dc.description.abstract | Purpose High rate of false-positive tests is a major obstacle to use human papillomavirus (HPV) detection as a diagnostic tool for high-grade squamous intraepithelial lesions or cervical cancer (HSIL+). We investigated whether type-specific viral load or physical state of HPV 16, 18, and 58 are useful biomarkers for HSIL+. Materials and Methods Type-specific viral loads of E6 and E2 genes in cervical cells from 240, 83, and 79 HPV 16-, 18-, and 58-infected women, respectively, were determined using real-time polymerase chain reaction. Viral loads were normalized to cellular DNA (copy/cell). Total and integrated viral loads and physical state were compared between HSIL+ and controls, and diagnostic value was determined using receiver operating characteristic analysis. Results Viral loads of HPV 16, 18, and 58 were significantly different in lesions in the same patho-logic grade. High type-specific total viral loads were significantly associated with HSIL+ (odds ratio [OK 14.065, 39.472, and 7.103 for HPV 16,18, and 58, respectively). High integrated viral load was related to HSIL+ in women with HPV 16 (OR, 8.242), and integrated state was associated with HSIL+ in women with HPV 18 (OR, 9.443). Type-specific total viral load was significantly associated with HSIL+ (area under curve, 0.914, 0.937, and 0.971 for HPV 16, 18, and 58, respectively), indicating an excellent performance in detecting HSIL+. Conclusion Type-specific total viral load may be a powerful diagnostic marker for HSIL+ in HPV 16-, 18-, and 58-infected HSIL+ lesions. If demonstrated in all other high-risk HPV types, this method can lead to a paradigm shift in the strategy of equivocal cytologic abnormalities. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한암학회 | - |
dc.title | Type-Specific Viral Load and Physical State of HPV Type 16, 18, and 58 as Diagnostic Biomarkers for High-Grade Squamous Intraepithelial Lesions or Cervical Cancer | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4143/crt.2019.152 | - |
dc.identifier.scopusid | 2-s2.0-85083389591 | - |
dc.identifier.wosid | 000526123000007 | - |
dc.identifier.bibliographicCitation | Cancer Research and Treatment, v.52, no.2, pp 396 - 405 | - |
dc.citation.title | Cancer Research and Treatment | - |
dc.citation.volume | 52 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 396 | - |
dc.citation.endPage | 405 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002577141 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | HUMAN-PAPILLOMAVIRUS TYPE-16 | - |
dc.subject.keywordPlus | RISK HUMAN-PAPILLOMAVIRUS | - |
dc.subject.keywordPlus | PREDICT RISK | - |
dc.subject.keywordPlus | DNA LOAD | - |
dc.subject.keywordPlus | NEOPLASIA | - |
dc.subject.keywordPlus | WOMEN | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | PREVALENCE | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Human papillomavirus | - |
dc.subject.keywordAuthor | Viral load | - |
dc.subject.keywordAuthor | High-grade squamous intraepithelial lesions | - |
dc.subject.keywordAuthor | Cervical cancer | - |
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