Soya protein isolate-polyethylene oxide electrospun nanofiber membrane with bone marrow-derived mesenchymal stem cell for enhanced bone regeneration
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun-Jung | - |
dc.contributor.author | Abueva, Celine D. G. | - |
dc.contributor.author | Padalhin, Andrew R. | - |
dc.contributor.author | Lee, Byong-Tael | - |
dc.date.accessioned | 2021-08-11T08:37:30Z | - |
dc.date.available | 2021-08-11T08:37:30Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 0885-3282 | - |
dc.identifier.issn | 1530-8022 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3070 | - |
dc.description.abstract | In this study, we prepared an electrospun nanofiber membrane from soya protein isolate (SPI) and polyethylene oxide (PEO) loaded with rat bone marrow-derived mesenchymal stem cells (rBMSC), as a cell-scaffold approach to enhance bone regeneration. Different ratios of SPI:PEO (7:0, 7:1, 7:3, 7:5, and 0:7) was investigated to obtain uniform nanofibers, and crosslinked with EDC/NHS to stabilize the membranes. SPI/PEO membrane (7:3) was found to create more uniform and stable nanofibers at a flow rate of 9 mu L/min, spun in a cylindrical collector rotating at 350 r/min, 23 kV DC voltage, and needle tip to collector distance of 13 cm. The loaded rBMSC were pre-differentiated to ensure commitment towards osteoblastic lineage. The SPI/PEO electrospun nanofiber membranes were successful in allowing for cell attachment and growth of the rBMSC and was further investigated in vivo using a rat skull defect model. New bone formation was observed for the optimized SPI/PEO electrospun nanofiber membrane (7:3) with and without rBMSC, but with faster new bone formation for SPI/PEO electrospun nanofiber membrane loaded with rBMSC as compared to SPI/PEO electrospun nanofiber membrane only and control (defect only). | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SAGE Publications | - |
dc.title | Soya protein isolate-polyethylene oxide electrospun nanofiber membrane with bone marrow-derived mesenchymal stem cell for enhanced bone regeneration | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1177/0885328219891614 | - |
dc.identifier.scopusid | 2-s2.0-85077186810 | - |
dc.identifier.wosid | 000501050700001 | - |
dc.identifier.bibliographicCitation | Journal of Biomaterials Applications, v.34, no.8, pp 1142 - 1149 | - |
dc.citation.title | Journal of Biomaterials Applications | - |
dc.citation.volume | 34 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1142 | - |
dc.citation.endPage | 1149 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | TISSUE REGENERATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | FIBERS | - |
dc.subject.keywordPlus | BIOCOMPOSITES | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordAuthor | Electrospun nanofiber membrane | - |
dc.subject.keywordAuthor | soya | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.subject.keywordAuthor | bone | - |
dc.subject.keywordAuthor | membrane scaffold | - |
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