A novel decellularized skeletal muscle-derived ECM scaffolding system for in situ muscle regeneration
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyeongjin | - |
dc.contributor.author | Ju, Young Min | - |
dc.contributor.author | Kim, Ickhee | - |
dc.contributor.author | Elsangeedy, Ebrahim | - |
dc.contributor.author | Lee, Joon Ho | - |
dc.contributor.author | Yoo, James J. | - |
dc.contributor.author | Atala, Anthony | - |
dc.contributor.author | Lee, Sang Jin | - |
dc.date.accessioned | 2021-08-11T08:38:47Z | - |
dc.date.available | 2021-08-11T08:38:47Z | - |
dc.date.issued | 2020-01-15 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.issn | 1095-9130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3175 | - |
dc.description.abstract | The cell-based tissue engineering strategies have gained attention in restoring normal tissue function after skeletal muscle injuries; however, these approaches require a donor tissue biopsy and extensive cell expansion process prior to implantation. In order to avoid this limitation, we developed a novel cell-free muscle-specific scaffolding system that consisted of a skeletal muscle-derived decellularized extracellular matrix (dECM) and a myogenic factor, insulin growth factor-1 (IGF-1). Rheological, morphological, and biological properties of this muscle-specific scaffold (IGF-1/dECM) as well as collagen and dECM scaffolds were examined. The cell viability in all scaffolds had over 90% at 1, 3, and 7 days in culture. The cell proliferation in the IGF-1/dECM was significantly increased when compared with other groups. More importantly, the IGF-1/dECM strongly supported the myogenic differentiation in the scaffold as confirmed by myosin heavy chain (MHC) immunofluorescence. We also investigated the feasibility in a rabbit tibialis anterior (TA) muscle defect model. The IGF1/dECM had a significantly greater number of myofibers when compared to both collagen and dECM groups at 1 and 2 months after implantation. We demonstrated that this novel muscle-specific scaffolding system could effectively promote the muscle tissue regeneration in situ. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | A novel decellularized skeletal muscle-derived ECM scaffolding system for in situ muscle regeneration | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.ymeth.2019.06.027 | - |
dc.identifier.scopusid | 2-s2.0-85068268885 | - |
dc.identifier.wosid | 000510529900009 | - |
dc.identifier.bibliographicCitation | Methods, v.171, pp 77 - 85 | - |
dc.citation.title | Methods | - |
dc.citation.volume | 171 | - |
dc.citation.startPage | 77 | - |
dc.citation.endPage | 85 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | CARTILAGE MATRIX | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INJURIES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | VITRO | - |
dc.subject.keywordAuthor | Decellularization | - |
dc.subject.keywordAuthor | Skeletal muscle | - |
dc.subject.keywordAuthor | Extracellular matrix | - |
dc.subject.keywordAuthor | Insulin-like growth factor 1 | - |
dc.subject.keywordAuthor | Tissue engineering | - |
dc.subject.keywordAuthor | In situ tissue regeneration | - |
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