Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse
DC Field | Value | Language |
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dc.contributor.author | Kwon, Mi-Hye | - |
dc.contributor.author | Song, Kang-Moon | - |
dc.contributor.author | Limanjaya, Anita | - |
dc.contributor.author | Choi, Min-Ji | - |
dc.contributor.author | Ghatak, Kalyan | - |
dc.contributor.author | Nhat Minh Nguyen | - |
dc.contributor.author | Ock, Jiyeon | - |
dc.contributor.author | Yin, Guo Nan | - |
dc.contributor.author | Kang, Ju-Hee | - |
dc.contributor.author | Lee, Man Ryul | - |
dc.contributor.author | Gho, Yong Song | - |
dc.contributor.author | Ryu, Ji-Kan | - |
dc.contributor.author | Suh, Jun-Kyu | - |
dc.date.accessioned | 2021-08-11T08:43:57Z | - |
dc.date.available | 2021-08-11T08:43:57Z | - |
dc.date.issued | 2019-12-27 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3738 | - |
dc.description.abstract | Extracellular vesicles (EVs) have attracted particular interest in various fields of biology and medicine. However, one of the major hurdles in the clinical application of EV-based therapy is their low production yield. We recently developed cell-derived EV-mimetic nanovesicles (NVs) by extruding cells serially through filters with diminishing pore sizes (10, 5, and 1 mu m). Here, we demonstrate in diabetic mice that embryonic stem cell (ESC)-derived EV-mimetic NVs (ESC-NVs) completely restore erectile function (similar to 96% of control values) through enhanced penile angiogenesis and neural regeneration in vivo, whereas ESC partially restores erectile function (similar to 77% of control values). ESC-NVs promoted tube formation in primary cultured mouse cavernous endothelial cells and pericytes under high-glucose condition in vitro; and accelerated microvascular and neurite sprouting from aortic ring and major pelvic ganglion under high-glucose condition ex vivo, respectively. ESC-NVs enhanced the expression of angiogenic and neurotrophic factors (hepatocyte growth factor, angiopoietin-1, nerve growth factor, and neurotrophin-3), and activated cell survival and proliferative factors (Akt and ERK). Therefore, it will be a better strategy to use ESC-NVs than ESCs in patients with erectile dysfunction refractory to pharmacotherapy, although it remains to be solved for future clinical application of ESC. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/s41598-019-54431-4 | - |
dc.identifier.scopusid | 2-s2.0-85077322468 | - |
dc.identifier.wosid | 000509327800018 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.9 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | INTRACAVERNOUS DELIVERY | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | TIE2 | - |
dc.subject.keywordPlus | ANGIOPOIETIN-1 | - |
dc.subject.keywordPlus | IMPROVE | - |
dc.subject.keywordAuthor | embryonic stem cell | - |
dc.subject.keywordAuthor | exosome | - |
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