Volume Adaptation Controls Stem Cell Mechanotransduction
DC Field | Value | Language |
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dc.contributor.author | Major, Luke G. | - |
dc.contributor.author | Holle, Andrew W. | - |
dc.contributor.author | Young, Jennifer L. | - |
dc.contributor.author | Hepburn, Matt S. | - |
dc.contributor.author | Jeong, Kwanghee | - |
dc.contributor.author | Chin, Ian L. | - |
dc.contributor.author | Sanderson, Rowan W. | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Aman, Zachary M. | - |
dc.contributor.author | Kennedy, Brendan F. | - |
dc.contributor.author | Hwang, Yongsung | - |
dc.contributor.author | Han, Dong-Wook | - |
dc.contributor.author | Park, Hyun Woo | - |
dc.contributor.author | Guan, Kun-Liang | - |
dc.contributor.author | Spatz, Joachim P. | - |
dc.contributor.author | Choi, Yu Suk | - |
dc.date.accessioned | 2021-08-11T08:43:59Z | - |
dc.date.available | 2021-08-11T08:43:59Z | - |
dc.date.issued | 2019-12-11 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.issn | 1944-8252 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3745 | - |
dc.description.abstract | Recent studies have found discordant mechanosensitive outcomes when comparing 2D and 3D, highlighting the need for tools to study mechanotransduction in 3D across a wide spectrum of stiffness. A gelatin methacryloyl (GelMA) hydrogel with a continuous stiffness gradient ranging from 5 to 38 kPa was developed to recapitulate physiological stiffness conditions. Adipose-derived stem cells (ASCs) were encapsulated in this hydrogel, and their morphological characteristics and expression of both mechanosensitive proteins (Lamin A, YAP, and MRTFa) and differentiation markers (PPAR gamma and RUNX2) were analyzed. Low-stiffness regions (similar to 8 kPa) permitted increased cellular and nuclear volume and enhanced mechanosensitive protein localization in the nucleus. This trend was reversed in high stiffness regions (similar to 30 kPa), where decreased cellular and nuclear volumes and reduced mechanosensitive protein nuclear localization were observed. Interestingly, cells in soft regions exhibited enhanced osteogenic RUNX2 expression, while those in stiff regions upregulated the adipogenic regulator PPAR gamma, suggesting that volume, not substrate stiffness, is sufficient to drive 3D stem cell differentiation. Inhibition of myosin II (Blebbistatin) and ROCK (Y-27632), both key drivers of actomyosin contractility, resulted in reduced cell volume, especially in low-stiffness regions, causing a decorrelation between volume expansion and mechanosensitive protein localization. Constitutively active and inactive forms of the canonical downstream mechanotransduction effector TAZ were stably transfected into ASCs. Activated TAZ resulted in higher cellular volume despite increasing stiffness and a consistent, stiffness-independent translocation of YAP and MRTFa into the nucleus. Thus, volume adaptation as a function of 3D matrix stiffness can control stem cell mechanotransduction and differentiation. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Volume Adaptation Controls Stem Cell Mechanotransduction | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsami.9b19770 | - |
dc.identifier.scopusid | 2-s2.0-85076022315 | - |
dc.identifier.wosid | 000502689000015 | - |
dc.identifier.bibliographicCitation | ACS Applied Materials & Interfaces, v.11, no.49, pp 45520 - 45530 | - |
dc.citation.title | ACS Applied Materials & Interfaces | - |
dc.citation.volume | 11 | - |
dc.citation.number | 49 | - |
dc.citation.startPage | 45520 | - |
dc.citation.endPage | 45530 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordPlus | STIFFNESS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | RIGIDITY | - |
dc.subject.keywordPlus | YAP/TAZ | - |
dc.subject.keywordAuthor | cellular volume | - |
dc.subject.keywordAuthor | stiffness gradient | - |
dc.subject.keywordAuthor | mechanotransduction | - |
dc.subject.keywordAuthor | stem cell differentiation | - |
dc.subject.keywordAuthor | extracellular matrix | - |
dc.subject.keywordAuthor | mechanobiology | - |
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