Enhanced decellularization technique of porcine dermal ECM for tissue engineering applications
DC Field | Value | Language |
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dc.contributor.author | Ventura, Reiza D. | - |
dc.contributor.author | Padalhin, Andrew R. | - |
dc.contributor.author | Park, Chan Mi | - |
dc.contributor.author | Lee, Byong Taek | - |
dc.date.accessioned | 2021-08-11T09:23:51Z | - |
dc.date.available | 2021-08-11T09:23:51Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 0928-4931 | - |
dc.identifier.issn | 1873-0191 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4132 | - |
dc.description.abstract | Effective removal of cellular components while retaining extracellular matrix (ECM) proteins is the ultimate goal of decellularization. The aim of this study is to produce a decellularized ECM with highly preserved ECM proteins and to determine the effect of isopropanol as a decellularization solvent on the characteristics of the decellularized porcine skin. Two different protocols were used for porcine skin decellularization. Protocol 1 consisted of Triton-X and sodium dodecyl sulfate (SDS) in water while protocol 2 consisted of Triton-X and SDS in 70% isopropanol. After decellularization, DNA components decreased significantly in protocol 2 with lower amount of lipid content and higher ECM proteins such as collagen (92.91 +/- 9.02 mu g/mg sample), alpha-elastin (142.32 +/- 6.74 mu g/mg sample) and sulfated glycosaminoglycan (sGAG; 7.44 +/- 1.30 mu g/mg sample) compared with protocol 1 ECM. Higher amount of vascular endothelial growth factor (VEGF; 11.26 +/- 0.44 pg/mg sample) content was quantified in protocol 2 compared with protocol 1 while higher trace amount of bone morphogenic protein 2 (BMP-2; 0.28 +/- 0.04 pg/mg sample) was also observed in protocol 2 compared with protocol 1. Protocol 2 ECM did not significantly affect the cell viability and exhibited no cytotoxicity when exposed to three different cell lines: L929 fibroblast cells, MC3T3-E1 pre-osteoblast cells, and rat mesenchymal stem cells (BMSC). Subcutaneous implantation after 7 and 21 days revealed higher cell infiltration in protocol 2 ECM and enhanced neovascularization. Isopropanol/surfactants proved to be effective in cell and lipid removal during decellularization while preserving the higher amount of ECM proteins. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Enhanced decellularization technique of porcine dermal ECM for tissue engineering applications | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.msec.2019.109841 | - |
dc.identifier.scopusid | 2-s2.0-85067610193 | - |
dc.identifier.wosid | 000487569300038 | - |
dc.identifier.bibliographicCitation | Materials Science and Engineering: C, v.104 | - |
dc.citation.title | Materials Science and Engineering: C | - |
dc.citation.volume | 104 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | SMALL-INTESTINAL SUBMUCOSA | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | ADIPOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | BIOMATERIAL | - |
dc.subject.keywordPlus | SCAFFOLD | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordAuthor | Extracellular matrix | - |
dc.subject.keywordAuthor | Porcine skin | - |
dc.subject.keywordAuthor | Isopropanol | - |
dc.subject.keywordAuthor | Decellularization | - |
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