MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathy
DC Field | Value | Language |
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dc.contributor.author | Jang, Sun Young | - |
dc.contributor.author | Chae, Min Kyung | - |
dc.contributor.author | Lee, Joon H. | - |
dc.contributor.author | Lee, Eun Jig | - |
dc.contributor.author | Yoon, Jin Sook | - |
dc.date.accessioned | 2021-08-11T09:24:44Z | - |
dc.date.available | 2021-08-11T09:24:44Z | - |
dc.date.issued | 2019-08-15 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4311 | - |
dc.description.abstract | Background To investigate the role of microRNA (miR)-27a and miR-27b in adipogenesis in an in vitro model of Graves' orbitopathy (GO). Methods Orbital fat tissues were harvested from GO and non-GO participants for primary orbital fibro-blast cultures. The expression levels of miR-27a and miR-27b between GO and non-GO orbital fat tissues were compared. During adipogenesis of GO orbital fibroblasts, the expression levels of miR-27a and miR-27b were determined, and the effects of mimics of miR-27a and miR-27b transfection on adipogenesis of GO orbital fibroblast were investigated. Results Real time-polymerase chain reaction showed significantly more decreases in miR-27a and miR-27b levels in orbital fat tissues in GO participants than in non-GO participants (p < 0.05). The expression of both miR-27a and miR-27b was highest in orbital fibroblasts at day 0 and declined gradually after the induction of adipogenic differentiation. The expression levels of PPAR gamma, CCAAT/enhancer binding protein (C/EBP)alpha and C/EBP beta were decreased and Oil Red O-stained lipid droplets were lower in GO orbital fibroblasts transfected with miR-27a and miR-27b mimics than in negative controls. Conclusions Our results indicated that miR-27a and miR-27b inhibited adipogenesis in orbital fibroblasts from GO patients. Further studies are required to examine the potential of miR-27a and miR-27b as targets for therapeutic strategies. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Public Library of Science | - |
dc.title | MicroRNA-27 inhibits adipogenic differentiation in orbital fibroblasts from patients with Graves' orbitopathy | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1371/journal.pone.0221077 | - |
dc.identifier.scopusid | 2-s2.0-85070772785 | - |
dc.identifier.wosid | 000485017200068 | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.14, no.8 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 14 | - |
dc.citation.number | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | FAT DECOMPRESSION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | QUERCETIN | - |
dc.subject.keywordPlus | DIPLOPIA | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | Graves' ophthalmopathy | - |
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