Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells
- Authors
- Rhee, Yong-Hee; Puspita, Lesly; Sulistio, Yanuar Alan; Kim, Seung Won; Vidyawan, Vincencius; Elvira, Rosalie; Chang, Mi-Yoon; Shim, Jae-won; Lee, Sang-Hun
- Issue Date
- 3-Jul-2019
- Publisher
- Nature Publishing Group
- Keywords
- Neuronal differentiation
- Citation
- Molecular Therapy, v.27, no.7, pp 1299 - 1312
- Pages
- 14
- Journal Title
- Molecular Therapy
- Volume
- 27
- Number
- 7
- Start Page
- 1299
- End Page
- 1312
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4389
- DOI
- 10.1016/j.ymthe.2019.04.011
- ISSN
- 1525-0016
1525-0024
- Abstract
- In this study, we found that undifferentiated human pluripotent stem cells (hPSCs; up to 30% of total cells) present in the cultures of neural stem or precursor cells (NPCs) completely disappeared within several days when cultured under neural differentiation culture conditions. Intriguingly, the disappearance of undifferentiated cells was not due to cell death but was instead mediated by neural conversion of hPSCs. Based on these findings, we propose pre-conditioning of donor NPC cultures under terminal differentiation culture conditions as a simple but efficient method of eliminating undifferentiated cells to treat neurologic disorders. In addition, we could establish a new neural differentiation protocol, in which undifferentiated hPSCs co-cultured with NPCs become differentiated neurons or NPCs in an extremely efficient, fast, and reproducible manner across the hESC and human-induced pluripotent stem cell (hiPSC) lines.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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