The role of Wnt/beta-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, In Hwan | - |
dc.contributor.author | Park, Seong Jun | - |
dc.contributor.author | Lee, Si Hyung | - |
dc.contributor.author | Nah, Seung Kwan | - |
dc.contributor.author | Park, Ha Yan | - |
dc.contributor.author | Yang, Jin Young | - |
dc.contributor.author | Madrakhimov, Sanjar Batirovich | - |
dc.contributor.author | Lyu, Jungmook | - |
dc.contributor.author | Park, Tae Kwann | - |
dc.date.accessioned | 2021-08-11T09:44:25Z | - |
dc.date.available | 2021-08-11T09:44:25Z | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 0268-8921 | - |
dc.identifier.issn | 1435-604X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/4628 | - |
dc.description.abstract | To investigate the role of Wnt/-catenin signaling pathway in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE) after laser photocoagulation. After differentiation of RPE cells from hiPSCs, laser photocoagulation was performed. Activation of Wnt/-catenin signaling at days 1 and 5 after laser photocoagulation was evaluated by expression of -catenin. Cell proliferation and alteration in cell-to-cell contact at day 5 after laser photocoagulation with or without Dickkopf-1 (Dkk-1) treatment were studied using ethynyl-2-deoxyuridine (EdU) assay and zonula occludens-1 (ZO-1) expression analysis, respectively. The mRNA levels of Wnt genes at day 5 after laser photocoagulation were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Activation of Wnt/-catenin signaling at days 1 and 5 after laser photocoagulation was confirmed by -catenin accumulation in the cytoplasm and nucleus of hiPSC-RPE. Many EdU-positive cells also expressed -catenin, and the number of EdU-positive cells was decreased at day 5 after laser photocoagulation after Dkk-1 treatment, indicating that Wnt/-catenin signaling mediated hiPSC-RPE proliferation. ZO-1 expression was not decreased with Dkk-1 treatment at day 5 after laser photocoagulation, indicating that Wnt/-catenin signaling mediated hiPSC-RPE restoration. At day 5, after laser photocoagulation, mRNA levels of Wnt2b, Wnt3, Wnt5a, Wnt7a, and Wnt10b were increased. Wnt/-catenin signaling has a crucial role in restoration of hiPSC-RPE proliferation after laser photocoagulation. Manipulation of Wnt/-catenin signaling while elucidating the underlying mechanisms of RPE restoration might have a therapeutic potential in retinal degenerative diseases. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer Verlag | - |
dc.title | The role of Wnt/beta-catenin signaling in the restoration of induced pluripotent stem cell-derived retinal pigment epithelium after laser photocoagulation | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1007/s10103-018-2631-5 | - |
dc.identifier.scopusid | 2-s2.0-85053800174 | - |
dc.identifier.wosid | 000461371500017 | - |
dc.identifier.bibliographicCitation | Lasers in Medical Science, v.34, no.3, pp 571 - 581 | - |
dc.citation.title | Lasers in Medical Science | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 571 | - |
dc.citation.endPage | 581 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | RPE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordAuthor | Wnt/beta-catenin signaling pathway | - |
dc.subject.keywordAuthor | Induced pluripotent stem cell | - |
dc.subject.keywordAuthor | Retinal pigment epithelium | - |
dc.subject.keywordAuthor | Laser photocoagulation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.